Fluorescence-guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic nude mouse model.
Fluorescence-guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic nude mouse model.
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DOI：
10.1016/j.jss.2012.08.052
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发表时间：
2013-01
影响因子：
2.2
通讯作者：
Bouvet, Michael
中科院分区：
文献类型：
--
作者：
Metildi, Cristina A.;Kaushal, Sharmeela;Snyder, Cynthia S.;Hoffman, Robert M.;Bouvet, Michael
关键词：
我们询问荧光引导手术（FGS）是否可以改善荧光原位裸鼠人结肠癌模型的手术结果。我们建立了表达荧光蛋白的人结肠癌的荧光原位小鼠模型。肿瘤在强光手术（BLS）或FGS下切除。使用OV-100小动物成像系统获得术前和术后图像，以评估手术切除范围。与BLS组中58%的小鼠相比，接受FGS的所有原发性肿瘤小鼠均完全切除（p=0.001）。与BLS相比，FGS导致复发率降低（33% vs 62%，p=0.049），无病中位生存期从9周延长至>36周。中位总生存期从BLS组的16周增加到FGS组的31周。FGS导致67%的小鼠治愈（手术后>6个月时存活，无肿瘤证据），而接受BLS的小鼠仅为37%（p=0.049）。FGS显著改善了人结肠癌原位裸鼠模型的手术结局。目前的研究可以通过各种有效的荧光标记肿瘤的方法转化为临床。
We inquired if fluorescence-guided surgery (FGS) could improve surgical outcomes in fluorescent orthotopic nude mouse models of human colon cancer. We established fluorescent orthotopic mouse models of human colon cancer expressing a fluorescent protein. Tumors were resected under bright light surgery (BLS) or FGS. Pre- and post-operative images with the OV-100 Small Animal Imaging System were obtained to assess extent of surgical resection. All mice with primary tumor which had undergone FGS had complete resection as compared to 58% of mice in the BLS group (p=0.001). FGS resulted in decreased recurrence compared to BLS (33% vs 62%, p=0.049), and lengthened disease-free median survival from 9 weeks to >36 weeks. The median overall survival increased from 16 weeks in the BLS group to 31 weeks in the FGS group. FGS resulted in a cure in 67% of mice (alive without evidence of tumor at >6 months post-surgery) compared to only 37% of mice which underwent BLS (p=0.049). Surgical outcomes in orthotopic nude mouse models of human colon cancer were signficantly improved with FGS. The current study can be translated to the clinic by various effective methods of fluorescently labeling tumors.
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影响因子：
--
作者：
Tran Cao HS;Kaushal S;Metildi CA;Menen RS;Lee C;Snyder CS;Messer K;Pu M;Luiken GA;Talamini MA;Hoffman RM;Bouvet M
通讯作者：
Bouvet M
影响因子：
3.4
作者：
Hohenberger, W.;Weber, K.;Merkel, S.
通讯作者：
Merkel, S.
影响因子：
3.5
作者：
Kennedy, MD;Jallad, KN;Low, PS
通讯作者：
Low, PS
影响因子：
3.2
作者：
Kaushal, Sharmeela;McElroy, Michele K.;Luiken, George A.;Talamini, Mark A.;Moossa, A. R.;Hoffman, Robert M.;Bouvet, Michael
通讯作者：
Bouvet, Michael
影响因子：
4.6
作者：
Wagner, Oliver J.;Louie, Brian E.;Farivar, Alexander S.
通讯作者：
Farivar, Alexander S.