Investigating the Effects of Dehydrated Human Amnion-Chorion Membrane on Periodontal Healing.
Investigating the Effects of Dehydrated Human Amnion-Chorion Membrane on Periodontal Healing.
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DOI：
10.3390/biom12060857
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发表时间：
2022-06-20
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
每种生长因子（GF）都有不同的作用和目标，在牙周愈合中起着至关重要的作用。脱水人羊膜-绒毛膜（dHACM）含有多种GFs，已被用于促进伤口愈合。本研究的目的是通过体外和体内实验的方法来评估ddhacm对牙周愈合的影响。在大鼠身上建立标准化的牙周缺损。将缺陷随机分为未填充、填充羟丙基纤维素（HPC）和dHACM+HPC三组。术后2周和4周，通过显微计算机断层扫描（micro-CT）、组织学和免疫组织化学分析牙周愈合情况。将大鼠门牙牙周韧带源性细胞（pdlc）与dHACM提取物进行体外培养。采用WST-1和创面愈合试验观察细胞增殖和迁移情况。在体内，2周时显微ct检查显示dHACM+HPC组新骨形成增强。4周时，dHACM+HPC组血管内皮生长因子（VEGF）阳性细胞和α-平滑肌肌动蛋白（α-SMA）阳性血管比例显著高于未填充组。在体外，与对照组相比，100µg/mL的dHACM提取物显著增加了细胞的增殖和迁移。这些结果表明，ddhm中含有的GFs可促进pdlc的增殖、迁移和血管生成，从而促进牙周愈合。
Each growth factor (GF) has different effects and targets, and plays a critical role in periodontal healing. Dehydrated human amnion-chorion membrane (dHACM) contains various GFs and has been used to enhance wound healing. The purpose of this study was to evaluate the effects of dHACM on periodontal healing, using in vitro and in vivo experimental approaches. Standardized periodontal defects were created in rats. The defects were randomly divided into three groups: Unfilled, filled with hydroxypropyl cellulose (HPC), and dHACM+HPC. At 2 and 4 weeks postoperatively, periodontal healing was analyzed by microcomputed tomography (micro-CT), and histological and immunohistochemical analyses. In vitro, periodontal ligament-derived cells (PDLCs) isolated from rat incisors were incubated with dHACM extract. Cell proliferation and migration were evaluated by WST-1 and wound healing assay. In vivo, micro-CT examination at 2 weeks revealed enhanced formation of new bone in the dHACM+HPC group. At 4 weeks, the proportions of vascular endothelial growth factor (VEGF)-positive cells and α-smooth muscle actin (α-SMA)-positive blood vessels in the dHACM+HPC group were significantly greater than those in the Unfilled group. In vitro, dHACM extracts at 100 µg/mL significantly increased cell proliferation and migration compared with control. These findings suggest that GFs contained in dHACM promote proliferation and migration of PDLCs and angiogenesis, which lead to enhanced periodontal healing.
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