Antibacterial activity of endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae).
Antibacterial activity of endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae).
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DOI：
10.3389/fmicb.2015.00350
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发表时间：
2015
影响因子：
5.2
通讯作者：
Lima VL
中科院分区：
文献类型：
--
作者：
Dos Santos IP;da Silva LC;da Silva MV;de Araújo JM;Cavalcanti Mda S;Lima VL
内生真菌是从产自巴西的药用植物苦苣苔（Indigofera suffruticosa Miller）的健康叶片中分离出来的，这种植物在民间医学中被用来治疗各种疾病。初步筛选出的65株内生真菌中，有18株真菌对至少一种微生物具有抑制活性，其中以球形黑孢菌（Nigrospora sphaerica, URM-6060）和黄斑拟盘多毛孢（拟盘多毛菌，URM-6061）效果最好。在液体培养基和半固体培养基中发酵后，只有N. sphaerica表现出抗菌活性（在马铃薯葡萄糖肉汤- pdb和半固体水稻培养基中）。下一步，从水稻培养基中制备甲醇提取物（NsME）和从PDB上清液中制备乙酸乙酯提取物（NsEAE），两者都对革兰氏阴性菌和革兰氏阳性菌具有抗菌活性。对金黄色葡萄球菌效果最好，对NsME的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）分别为1.56 mg/mL和6.25 mg/mL，对NsEAE的MIC和MBC分别为0.39 mg/mL和3.12 mg/mL。本研究首次报道了寄生于苦参叶内的内生真菌的抗菌活性，其中真菌N. sphaerica显示了产生具有药用潜力的生物活性物质的能力，这可能为寻找新的候选药物生物来源提供新的线索。
Endophytic fungi were isolated from healthy leaves of Indigofera suffruticosa Miller, a medicinal plant found in Brazil which is used in folk medicine to treat various diseases. Among 65 endophytic fungi isolated, 18 fungi showed activity against at least one tested microorganism in preliminary screening, and the best results were obtained with Nigrospora sphaerica (URM-6060) and Pestalotiopsis maculans (URM-6061). After fermentation in liquid media and in semisolid media, only N. sphaerica demonstrated antibacterial activity (in Potato Dextrose Broth-PDB and in semisolid rice culture medium). In the next step, a methanolic extract from rice culture medium (NsME) and an ethyl acetate extract (NsEAE) from the supernatant of PDB were prepared and both exhibited antimicrobial activity against Gram-negative and Gram-positive bacteria. The best result was observed against Staphylococcus aureus, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 1.56 mg/mL and 6.25 mg/mL, respectively, for NsME and MIC and MBC values of 0.39 mg/mL and 3.12 mg/mL, respectively, for NsEAE. This study is the first report about the antimicrobial activity of endophytic fungi residing in I. suffruticosa leaves, in which the fungus N. sphaerica demonstrated the ability to produce bioactive agents with pharmaceutical potential, and may provide a new lead in the pursuit of new biological sources of drug candidates.
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影响因子：
4.4
作者：
Araújo, WL;Marcon, J;Azevedo, JL
通讯作者：
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影响因子：
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作者：
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