Improved influenza vaccination in the skin using vaccine coated microneedles.
Improved influenza vaccination in the skin using vaccine coated microneedles.
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DOI：
10.1016/j.vaccine.2009.08.108
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发表时间：
2009-11-16
期刊：
影响因子：
5.5
通讯作者：
Prausnitz MR
中科院分区：
文献类型：
--
作者：
Kim YC;Quan FS;Yoo DG;Compans RW;Kang SM;Prausnitz MR
简单有效的疫苗接种方法可以降低疫苗可预防的流感感染的死亡率和发病率。在这项研究中，我们检查了使用微针贴片，以增加患者的覆盖率，通过可能的自我管理和增强疫苗免疫原性，通过靶向输送到皮肤。我们进行了一个详细的研究后，一个单一的流感疫苗接种到小鼠皮肤的保护性免疫反应与一种新的微针补丁，旨在促进简单和可靠的疫苗输送。与肌内注射相比，使用灭活病毒包被微针的皮肤接种提供了针对致死性攻击的上级保护，如肺中的有效病毒清除所证明的。详细的免疫学分析表明，病毒中和抗体的诱导以及增强的回忆性体液和细胞反应有助于通过微针接种皮肤来改善保护。这些发现表明，使用微针贴片在皮肤中接种疫苗可以提高保护性免疫力，并简化流感疫苗和其他疫苗的接种。
Easy and effective vaccination methods could reduce mortality and morbidity due to vaccine-preventable influenza infections. In this study, we examined the use of microneedle patches to increase patient coverage through possible self administration and enhance vaccine immunogenicity by targeted delivery to skin. We carried out a detailed study of protective immune responses after a single influenza vaccination to the skin of mice with a novel microneedle patch designed to facilitate simple and reliable vaccine delivery. Skin vaccination with inactivated virus-coated microneedles provided superior protection against lethal challenge compared to intramuscular injection as evidenced by effective virus clearance in lungs. Detailed immunologic analysis suggests that induction of virus neutralizing antibodies as well as enhanced anamnestic humoral and cellular responses contributed to improved protection by microneedle vaccination to the skin. These findings suggest that vaccination in the skin using a microneedle patch can improve protective immunity, and simplify delivery of influenza and possibly other vaccines.
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