Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells.
Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells.
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DOI：
10.1038/s41598-017-09059-7
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发表时间：
2017-08-17
影响因子：
4.6
通讯作者：
Goya GF
中科院分区：
文献类型：
--
作者：
Calatayud MP;Soler E;Torres TE;Campos-Gonzalez E;Junquera C;Ibarra MR;Goya GF
我们提供了水浴（WB）和磁热疗（MHT）外源加热对神经胶质微肿瘤模型影响的证据。为此，设计了 30-40nm 的磁性纳米粒子 (MNP)，以获得最大加热效率的粒径。所制备胶体的比功率吸收 (SPA) 值 (f = 560 kHz, H = 23.9 kA/m) (533–605 W/g) 在培养基中降至 98–279 W/g。对细胞内 MNP 分布的分析显示，囊泡捕获的 MNP 聚集体沿着细胞质扩散，以及附着在细胞膜上的大 (~0.5–0.9μm) 簇。 WB 和 MHT（T = 46 °C，持续 30 分钟）后，细胞活力约为 70%，4.5 小时后降至 20-25%，表明代谢过程参与细胞杀伤。 MHT 后的细胞结构分析显示，细胞膜出现显着损伤，这与 MNP 簇的位置相关，而在没有 MNP 的 WB 后，局部细胞损伤不太明显。尽管 WB 和 MHT 对细胞活力的热效应相似，但我们的结果表明，存在与细胞内空间中 MNP 的存在相关的额外细胞损伤机制。
We present evidence on the effects of exogenous heating by water bath (WB) and magnetic hyperthermia (MHT) on a glial micro-tumor phantom. To this, magnetic nanoparticles (MNPs) of 30–40 nm were designed to obtain particle sizes for maximum heating efficiency. The specific power absorption (SPA) values (f = 560 kHz, H = 23.9 kA/m) for as prepared colloids (533–605 W/g) dropped to 98–279 W/g in culture medium. The analysis of the intracellular MNPs distribution showed vesicle-trapped MNPs agglomerates spread along the cytoplasm, as well as large (~0.5–0.9 μm) clusters attached to the cell membrane. Immediately after WB and MHT (T = 46 °C for 30 min) the cell viability was ≈70% and, after 4.5 h, decreased to 20–25%, demonstrating that metabolic processes are involved in cell killing. The analysis of the cell structures after MHT revealed a significant damage of the cell membrane that is correlated to the location of MNPs clusters, while local cell damage were less noticeable after WB without MNPs. In spite of the similar thermal effects of WB and MHT on the cell viability, our results suggest that there is an additional mechanism of cell damage related to the presence of MNPs at the intracellular space.
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影响因子：
4.6
作者：
Chalopin, Yann;Bacri, Jean-Claude;Devaud, Martin
通讯作者：
Devaud, Martin
影响因子：
4.8
作者：
Fleige, G;Nolte, C;Zimmer, C
通讯作者：
Zimmer, C
振荡磁性纳米颗粒在杀死肿瘤细胞中的形态学作用。
影响因子：
--
作者：
Cheng D;Li X;Zhang G;Shi H
通讯作者：
Shi H
影响因子：
5.4
作者：
Alizadeh, Darya;Zhang, Leying;Hwang, Jungyeon;Schluep, Thomas;Badie, Behnam
通讯作者：
Badie, Behnam
影响因子：
15
作者：
Fortin, Jean-Paul;Wilhelm, Claire;Gazeau, Florence
通讯作者：
Gazeau, Florence