The effect of arginine on oral biofilm communities.
The effect of arginine on oral biofilm communities.
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DOI：
10.1111/omi.12044
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发表时间：
2014-03
影响因子：
3.7
通讯作者：
Burne RA
中科院分区：
文献类型：
--
作者：
Nascimento MM;Browngardt C;Xiaohui X;Klepac-Ceraj V;Paster BJ;Burne RA
口腔细菌通过精氨酸脱亚胺酶系统（ADS）产生的碱增加了口腔生物膜的pH值，并降低了发生龋损的风险。本研究验证了通过外源性来源增加口腔环境中精氨酸的可用性可增强唾液和牙菌斑中ADS活性水平的假设。在治疗前和治疗后，收集19名无龋（CF）个体（DMFT = 0）和19名有龋（CA）个体（DMFT ≥ 2）的唾液和龈上菌斑样本，包括使用含1.5%精氨酸的无氟牙膏或常规含氟牙膏，每天两次，持续4周。在基线、洗脱期后、治疗4周和治疗后2周，通过口服样品定量精氨酸产生的氨来测量ADS活性。基线时，CF斑块样本中的ADS活性水平高于CA个体（P = 0.048）。使用精氨酸牙膏显著增加CA患者菌斑中的ADS活性（P = 0.026）。用精氨酸牙膏治疗的CA的菌斑微生物谱显示细菌组成向更健康的群体转变，更类似于CF个体。因此，可以从含马槟榔碱的制剂中预期抗龋齿效果，这在很大程度上是由于ADS活性水平的增强和对口腔微生物组的组成的潜在有利修饰。
Alkali production by oral bacteria via the arginine deiminase system (ADS) increases the pH of oral biofilms and reduces the risk for development of carious lesions. This study tested the hypothesis that increased availability of arginine in the oral environment through an exogenous source enhances the ADS activity levels in saliva and dental plaque. Saliva and supra-gingival plaque samples were collected from 19 caries-free (CF) individuals (DMFT = 0) and 19 caries-active (CA) individuals (DMFT ≥ 2) before and after treatment, which comprised the use of a fluoride-free toothpaste containing 1.5% arginine, or a regular fluoride-containing toothpaste twice daily for 4 weeks. ADS activity was measured by quantification of ammonia produced from arginine by oral samples at baseline, after washout period, 4 weeks of treatment, and 2 weeks post-treatment. Higher ADS activity levels were observed in plaque samples from CF compared to those of CA individuals (P = 0.048) at baseline. The use of the arginine toothpaste significantly increased ADS activity in plaque of CA individuals (P = 0.026). The plaque microbial profiles of CA treated with the arginine toothpaste showed a shift in bacterial composition to a healthier community, more similar to that of CF individuals. Thus, an anti-caries effect may be expected from arginine-containing formulations due in large part to the enhancement of ADS activity levels and potential favorable modification to the composition of the oral microbiome.
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影响因子：
18.6
作者：
Paster, Bruce J.;Olsen, Ingar;Dewhirst, Floyd E.
通讯作者：
Dewhirst, Floyd E.
影响因子：
7.6
作者：
Nascimento, M. M.;Liu, Y.;Burne, R. A.
通讯作者：
Burne, R. A.
影响因子：
3
作者：
KLEINBERG, I
通讯作者：
KLEINBERG, I
影响因子：
3.6
作者：
PETERSON, S;WOODHEAD, J;CRALL, J
通讯作者：
CRALL, J
影响因子：
4.3
作者：
Colombo AP;Boches SK;Cotton SL;Goodson JM;Kent R;Haffajee AD;Socransky SS;Hasturk H;Van Dyke TE;Dewhirst F;Paster BJ
通讯作者：
Paster BJ