Fluorescence-labeled reporter gene in transgenic mice provides a useful tool for investigating cutaneous innervation.
Fluorescence-labeled reporter gene in transgenic mice provides a useful tool for investigating cutaneous innervation.
复制标题
DOI：
10.1177/0300985811414033
复制
发表时间：
2012-07
影响因子：
2.4
通讯作者：
Sundberg JP
中科院分区：
文献类型：
--
作者：
Webb CM;Cameron EM;Sundberg JP
B6.Cg-TG(Thy1-YFP)16Jars/J转基因小鼠在小鼠Thy1启动子的驱动下表达黄色荧光蛋白基因，该启动子标记运动神经元和感觉神经元，以便对单个神经进行跟踪。我们使用这些小鼠来识别皮肤中支配竖起毛发和肌腱膜肌肉的神经。用荧光标记的α-银环蛇毒素标记神经肌肉接头，证实皮肤神经中有黄色荧光蛋白的表达。冰冻和石蜡包埋的皮肤切片显示肌腱膜肌的神经支配。用抗GFP抗体标记的石蜡切片显示肌腱膜、竖毛肌和毛囊隆起周围的神经分布，后两种神经在直接荧光下看不到。
B6.Cg-Tg(Thy1-YFP)16Jrs/J transgenic mice were created to express the yellow fluorescent protein gene driven by a mouse Thy1 promoter which labeled motor and sensory neurons such that individual nerves could be followed. We used these mice to identify nerves in the skin that innervate the erector pili and panniculus carnosus muscle. Whole mounts demonstrated yellow fluorescent protein (YFP) expression in nerves of the skin which was confirmed by labeling the neuromuscular junction with fluorescinated α-bungarotoxin. Frozen and paraffin embedded skin sections revealed innervation of the panniculus carnosus muscle. Paraffin sections labeled with an anti-GFP antibody revealed innervation of the panniculus carnosus as well as the erector pili muscle and around the hair follicle bulge, the last two of which were not seen by direct fluorescence.
登录
查看更多内容
影响因子：
6.5
作者：
Pan, YA;Sanes, JR
通讯作者：
Sanes, JR
影响因子：
5.3
作者：
Zuo, Y;Lubischer, JL;Thompson, WJ
通讯作者：
Thompson, WJ
影响因子：
16.2
作者：
Feng, GP;Mellor, RH;Sanes, JR
通讯作者：
Sanes, JR
影响因子：
4.4
作者：
Yu, Charles Q.;Rosenblatt, Mark I.
通讯作者：
Rosenblatt, Mark I.
影响因子：
2.4
作者：
BOLON, B;MAYS, MBC
通讯作者：
MAYS, MBC