Peptaibols from two unidentified fungi of the order Hypocreales with cytotoxic, antibiotic, and anthelmintic activities.
Peptaibols from two unidentified fungi of the order Hypocreales with cytotoxic, antibiotic, and anthelmintic activities.
复制标题
DOI：
10.1002/psc.2425
复制
发表时间：
2012-08
影响因子：
2.1
通讯作者：
Oberlies, Nicholas H.
中科院分区：
文献类型：
--
作者：
Ayers, Sloan;Ehrmann, Brandie M.;Adcock, Audrey F.;Kroll, David J.;de Blanco, Esperanza J. Carcache;Shen, Qi;Swanson, Steven M.;Falkinham, Joseph O., III;Wani, Mansukh C.;Mitchell, Sheila M.;Pearce, Cedric J.;Oberlies, Nicholas H.
作为正在进行的丝状真菌抗癌线索研究的一部分，从Mycosynthetix文库（MSX 70741，肉座菌目，子囊菌门）中鉴定出活性培养物。该真菌提取物显示出对H460（人非小细胞肺癌）细胞系的细胞毒性活性，并且生物活性定向分级分离产生了肽酶1-12和哈茨酵母A（13）和B（14）。通过使用高能碰撞解离（HCD）在Thermo LTQ Orbitrap XL上获得的高分辨率MS/MS和通过高场NMR（950 MHz）促进1-12的结构解析。绝对构型通过Marveland对单个氨基酸的分析确定;通过开发10 min UPLC方法减少了此类分析所需的时间。在一系列生物测定中检查分离的肽（1-12）以及从相同目（15-17）的不同真菌（MSX 57715）分离和阐明的三种其它肽（沿着）的活性，包括细胞毒性、抗菌和驱虫活性。
As part of an ongoing investigation of filamentous fungi for anticancer leads, an active culture was identified from the Mycosynthetix library (MSX 70741, of the order Hypocreales, Ascomycota). The fungal extract exhibited cytotoxic activity against the H460 (human non-small cell lung carcinoma) cell line, and bioactivity-directed fractionation yielded peptaibols 1–12 and harzianums A (13) and B (14). Structure elucidation of 1–12 was facilitated by high-resolution MS/MS obtained on a Thermo LTQ Orbitrap XL using Higher-Energy Collisional Dissociation (HCD) and by high field NMR (950 MHz). The absolute configuration was determined by Marfey’s analysis of the individual amino acids; the time required for such analysis was decreased via the development of a 10 min UPLC method. The isolated peptaibols (1–12), along with three other peptaibols isolated and elucidated from a different fungus (MSX 57715) of the same Order (15–17), were examined for activity in a suite of biological assays, including those for cytotoxic, antibacterial, and anthelmintic activities.
登录
查看更多内容
影响因子：
5.1
作者：
Li, Chen;Lee, Dongho;Graf, Tyler N.;Phifer, Sharnelle S.;Nakanishi, Yuka;Riswan, Soedarsono;Setyowati, Fransisca M.;Saribi, Achmad M.;Soejarto, Djaja D.;Farnsworth, Norman R.;Falkinham, Joseph O., III;Kroll, David J.;Kinghorn, A. Douglas;Wani, Mansukh C.;Oberlies, Nicholas H.
通讯作者：
Oberlies, Nicholas H.
影响因子：
14.8
作者：
Kjer, Julia;Debbab, Abdessamad;Proksch, Peter
通讯作者：
Proksch, Peter
影响因子：
1.6
作者：
Lopez-Aroche, U.;Salinas-Sanchez, D. O.;Hernandez-Velazquez, V.
通讯作者：
Hernandez-Velazquez, V.
影响因子：
3.3
作者：
Leclerc, G;Rebuffat, S;Bodo, B
通讯作者：
Bodo, B
影响因子：
3.3
作者：
Chikanishi, T;Hasumi, K;Endo, A
通讯作者：
Endo, A