Magnetization transfer in liposome and proteoliposome samples that mimic the protein and lipid composition of myelin
Magnetization transfer in liposome and proteoliposome samples that mimic the protein and lipid composition of myelin
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模拟髓鞘质蛋白质和脂质组成的脂质体和蛋白脂质体样品中的磁化转移
DOI：
10.1002/nbm.4097
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发表时间：
2019
影响因子：
2.9
通讯作者：
Jerschow, Alexej
中科院分区：
文献类型：
--
作者：
Yang, Weiqi;Lee, Jae‐Seung;Leninger, Maureen;Windschuh, Johannes;Traaseth, Nathaniel J.;Jerschow, Alexej
虽然磁化转移（MT）已被广泛用于脑MRI，例如在脑炎症和多发性硬化症，MT效应的详细分子起源和蛋白质在MT中发挥的作用仍然不清楚。在这项工作中，使用蛋白脂质体模型系统来模拟髓鞘环境，并检查嵌入脂质体基质中的蛋白质、胆固醇、脑苷脂和鞘磷脂的作用。使用双量子滤波器实验确定交换参数。目的是确定1，2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱双层中糖苷、鞘磷脂、胆固醇和蛋白质对交换和MT的相对贡献。主要的发现是，β-糖苷产生了最强的交换效应，甚至比蛋白质的交换效应更明显。鞘磷脂（其在脂肪酸链的头部也具有可交换基团，尽管更接近脂质酰基链）和胆固醇仅显示出最小的转移。总体而言，提取的交换率似乎远小于通常假设的-OH和-NH基团。
Although magnetization transfer (MT) has been widely used in brain MRI, for example in brain inflammation and multiple sclerosis, the detailed molecular origin of MT effects and the role that proteins play in MT remain unclear. In this work, a proteoliposome model system was used to mimic the myelin environment and to examine the roles of protein, cholesterol, brain cerebrosides, and sphingomyelin embedded in the liposome matrix. Exchange parameters were determined using a double‐quantum filter experiment. The goal was to determine the relative contributions to exchange and MT of cerebrosides, sphingomyelin, cholesterol, and proteins in 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine bilayers. The main finding was that cerebrosides produced the strongest exchange effects, and that these were even more pronounced than those found for proteins. Sphingomyelin (which also has exchangeable groups at the head of the fatty acid chains, albeit closer to the lipid acyl chains) and cholesterol showed only minimal transfer. Overall, the extracted exchange rates appeared much smaller than commonly assumed for ‐OH and ‐NH groups.
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影响因子：
15
作者：
Eliav, U;Navon, G
通讯作者：
Navon, G
影响因子：
16.6
作者：
Gayen, Anindita;Banigan, James R.;Traaseth, Nathaniel J.
通讯作者：
Traaseth, Nathaniel J.
DOI：
--
发表时间：
2002-09
期刊：
AJNR. American journal of neuroradiology
影响因子：
--
作者：
Y. Ge;R. Grossman;J. Babb;Marcie L. Rabin;L. J. Mannon;D. Kolson
通讯作者：
Y. Ge;R. Grossman;J. Babb;Marcie L. Rabin;L. J. Mannon;D. Kolson
多发性硬化症磁化转移率与临床残疾的相关性
影响因子：
11.2
作者：
MD A. Gass;PhD G. J. Barker;Mrcp D. Kidd;Mrcp J. W. Thorpe;Dcr D. MacManus;Dcr A. Brennan;P. Tofts;A. J. DPhil;MD W. I Thompson;PhD D. H McDonald;MD Miller;G. Barker;J. Thorpe;P. Tofts;A. Thompson;Miller DH. Ann McDonald WI;Neurol
通讯作者：
Neurol
影响因子：
5.7
作者：
Janve, Vaibhav A.;Zu, Zhongliang;Yao, Song-Yi;Li, Ke;Zhang, Fang Lin;Wilson, Kevin J.;Ou, Xiawei;Does, Mark D.;Subramaniam, Sriram;Gochberg, Daniel F.
通讯作者：
Gochberg, Daniel F.