Molecular behavior of DNA origami in higher-order self-assembly.
Molecular behavior of DNA origami in higher-order self-assembly.
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DOI：
10.1021/ja106292x
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发表时间：
2010-09-29
影响因子：
15
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Li Z;Liu M;Wang L;Nangreave J;Yan H;Liu Y
基于DNA的自组装是一种独特的方法，用于实现更高阶的分子结构，这是由于DNA是一种可编程的信息编码聚合物。在过去的十年中，已经开发了两种主要类型的DNA纳米结构：具有小尺寸（通常高达~20 nm）的树枝状DNA瓦片和具有较大尺寸（高达~100 nm）的DNA折纸瓦片。在这里，我们的目的是确定参与组装DNA折纸超结构的重要因素。我们构建了一系列新的矩形DNA折纸瓦片，其中平行的DNA螺旋在沿DNA螺旋轴沿着观察时以锯齿形图案排列，这种设计是为了放松在原始平面矩形折纸瓦片中发现的内在全局扭曲。自关联锯齿形瓷砖被发现形成线性阵列在两个对角线方向，而平面瓷砖只在一个方向上表现出显着的增长。虽然一系列之字形瓦片被设计成促进二维阵列形成，但观察到的却是一维线性阵列和管状结构。我们发现，折纸单元瓦片的尺寸纵横比和瓦片间连接设计在确定最终产品中起着重要作用，正如原子力显微镜成像所揭示的那样。这项研究提供了从自组装DNA折纸瓦片形成高阶结构的见解，揭示了它们与具有较小尺寸的传统DNA瓦片相比的独特行为。
DNA-based self-assembly is a unique method for achieving higher-order molecular architectures made possible by the fact that DNA is a programmable information-coding polymer. In the past decade, two main types of DNA nanostructures have been developed: branch-shaped DNA tiles with small dimensions (commonly up to ~20 nm) and DNA origami tiles with larger dimensions (up to ~100 nm). Here we aimed to determine the important factors involved in the assembly of DNA origami superstructures. We constructed a new series of rectangular-shaped DNA origami tiles in which parallel DNA helices are arranged in a zigzag pattern when viewed along the DNA helical axis, a design conceived in order to relax an intrinsic global twist found in the original planar, rectangular origami tiles. Self-associating zigzag tiles were found to form linear arrays in both diagonal directions, while planar tiles showed significant growth in only one direction. Although the series of zigzag tiles were designed to promote two-dimensional array formation, one-dimensional linear arrays and tubular structures were observed instead. We discovered that the dimensional aspect ratio of the origami unit tiles and intertile connection design play important roles in determining the final products, as revealed by atomic force microscopy imaging. This study provides insight into the formation of higher-order structures from self-assembling DNA origami tiles, revealing their unique behavior in comparison with conventional DNA tiles having smaller dimensions.
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影响因子：
15
作者：
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影响因子：
15
作者：
Ke, YG;Liu, Y;Yan, H
通讯作者：
Yan, H