Carotenoid transporter CD36 expression depends on hypoxia-inducible factor-1α in mouse soleus muscles.
Carotenoid transporter CD36 expression depends on hypoxia-inducible factor-1α in mouse soleus muscles.
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DOI：
10.3164/jcbn.21-163
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发表时间：
2022-09
影响因子：
2.4
通讯作者：
Yamaji, Ryoichi
中科院分区：
文献类型：
--
作者：
Kitakaze, Tomoya;Sugihira, Takashi;Kameyama, Hiromichi;Maruchi, Asami;Kobayashi, Yasuyuki;Harada, Naoki;Yamaji, Ryoichi
膳食β-胡萝卜素会导致红色慢肌比目鱼肌肥大并防止肌肉萎缩，但不会影响白色快肌趾长伸肌 (EDL) 和腓肠肌。然而，目前尚不清楚为什么 β-胡萝卜素会在比目鱼肌中产生这些有益作用。为了解决这个问题，我们重点关注骨骼肌中的类胡萝卜素转运蛋白。在小鼠中，红色肌肉中的 Cd36 mRNA 水平高于白色肌肉中的 Cd36 mRNA 水平。 siRNA 介导的 CD36 敲除减少了 C2C12 肌管中 β-胡萝卜素的摄取。在比目鱼肌中，CD36 敲除抑制了 β-胡萝卜素诱导的肌肉质量增加。静脉注射缺氧标志物哌莫硝唑在比目鱼肌中比在 EDL 肌肉中产生更多的哌莫硝唑结合蛋白，并且比目鱼肌中缺氧诱导因子 1 (HIF-1) α 蛋白水平比 EDL 肌肉中更高。在C2C12肌管中，缺氧增加了CD36和HIF-1α在蛋白质和mRNA水平上的表达，而HIF-1α敲低则减少了缺氧诱导的Cd36 mRNA水平的增加。在比目鱼肌中，HIF-1α 敲低降低了 Cd36 mRNA 水平。这些结果表明CD36主要参与β-胡萝卜素诱导的小鼠比目鱼肌质量的增加。此外，我们证明即使在正常生理条件下，CD36 的表达也依赖于小鼠比目鱼肌中的 HIF-1α。
Dietary β-carotene induces muscle hypertrophy and prevents muscle atrophy in red slow-twitch soleus muscles, but not in white fast-twitch extensor digitorum longus (EDL) muscles and gastrocnemius muscles. However, it remains unclear why these beneficial effects of β-carotene are elicited in soleus muscles. To address this issue, we focused on carotenoid transporters in skeletal muscles. In mice, Cd36 mRNA levels were higher in red muscle than in white muscle. The siRNA-mediated knockdown of CD36 decreased β-carotene uptake in C2C12 myotubes. In soleus muscles, CD36 knockdown inhibited β-carotene-induced increase in muscle mass. Intravenous injection of the hypoxia marker pimonidazole produced more pimonidazole-bound proteins in soleus muscles than in EDL muscles, and the hypoxia-inducible factor-1 (HIF-1) α protein level was higher in soleus muscles than in EDL muscles. In C2C12 myotubes, hypoxia increased the expression of CD36 and HIF-1α at the protein and mRNA levels, and HIF-1α knockdown reduced hypoxia-induced increase in Cd36 mRNA level. In soleus muscles, HIF-1α knockdown reduced Cd36 mRNA level. These results indicate that CD36 is predominantly involved in β-carotene-induced increase in soleus muscle mass of mice. Furthermore, we demonstrate that CD36 expression depends on HIF-1α in the soleus muscles of mice, even under normal physiological conditions.
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影响因子：
3.7
作者：
Bloemberg D;Quadrilatero J
通讯作者：
Quadrilatero J
DOI：
10.1016/j.bbalip.2011.06.002
发表时间：
2012-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Harrison EH
通讯作者：
Harrison EH
影响因子：
1.6
作者：
Kitakaze, Tomoya;Harada, Naoki;Yamaji, Ryoichi
通讯作者：
Yamaji, Ryoichi
影响因子：
4.8
作者：
Mitani, Takakazu;Harada, Naoki;Yamaji, Ryoichi
通讯作者：
Yamaji, Ryoichi
影响因子：
5.6
作者：
Liu, Siqi;Yang, Dan;Zhou, Lei
通讯作者：
Zhou, Lei