Quantification of within- and between-farm dispersal of Culicoides biting midges using an immunomarking technique.
Quantification of within- and between-farm dispersal of Culicoides biting midges using an immunomarking technique.
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DOI：
10.1111/1365-2664.12875
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发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Carpenter S
中科院分区：
文献类型：
--
作者：
Sanders CJ;Harrup LE;Tugwell LA;Brugman VA;England M;Carpenter S
库蠓（双翅目，蠓科）是虫媒病毒的媒介，对经济和福利造成重大影响。库蠓传播的虫媒病毒在当地的传播很大程度上取决于受感染库蠓在农场间的移动。通过捕获-标记-再捕获（CMR）来研究库蠓的扩散行为是有问题的，因为这些昆虫的小尺寸和脆弱性（再捕获率低）可能导致死亡和捕获后行为的变化。为了解决库蠓使用CMR的问题，本研究利用卵清蛋白免疫标记技术来量化英格兰南部库蠓在农场内和农场间的传播。观察到库蠓在农场内和农场间的扩散。在22夜捕获的9058只库蠓中，收集到卵清蛋白阳性库蠓600只，包括作为虫媒病毒载体的12种，最大传播距离为3125 m。本研究首次提供了关于北欧潜在蓝舌病、施马伦贝格病和非洲马病病毒媒介在农场间传播的物种水平数据。高分辨率的气象数据确定了库蠓的逆风和顺风飞行。累计收集和气象资料表明，在1 km以上的飞行中，15.6%的飞行在处理区域的逆风方向，84%的飞行在下风方向。合成与应用。免疫标记的使用消除了在标记之前对昆虫收集的生存和行为的潜在不利影响，大大提高了对库蠓等小型和脆弱病媒物种传播潜力估计的分辨率和准确性。利用这一技术，根据气象条件（包括风向）对库蠓传播范围进行量化，将有助于改进库蠓传播的虫媒病毒的数据驱动传播建模，并将为应对库蠓入侵和暴发提供政策信息。免疫标记的使用消除了在标记之前对昆虫收集的生存和行为的潜在不利影响，大大提高了对库蠓等小型和脆弱病媒物种传播潜力估计的分辨率和准确性。利用这一技术，根据气象条件（包括风向）对库蠓传播范围进行量化，将有助于改进库蠓传播的虫媒病毒的数据驱动传播建模，并将为应对库蠓入侵和暴发提供政策信息。
Culicoides biting midges (Diptera, Ceratopogonidae) are vectors of arboviruses that cause significant economic and welfare impact. Local‐scale spread of Culicoides‐borne arboviruses is largely determined by the between‐farm movement of infected Culicoides. Study of the dispersal behaviour of Culicoides by capture–mark–recapture (CMR) is problematic due to the likelihood of mortality and changes in behaviour upon capture caused by the small size and fragility of these insects, evidenced by low recapture rates. To counter the problem of using CMR with Culicoides, this study utilised an ovalbumin immunomarking technique to quantify the within‐ and between‐farm dispersal of Culicoides in southern England. Both within‐ and between‐farm dispersal of Culicoides was observed. Of the 9058 Culicoides collected over 22 nights of trapping, 600 ovalbumin‐positive Culicoides, of 12 species including those implicated as arbovirus vectors, were collected with a maximum dispersal distance of 3125 m. This study provides the first species‐level data on the between‐farm dispersal of potential bluetongue, Schmallenberg and African horse sickness virus vectors in northern Europe. High‐resolution meteorological data determined upwind and downwind flight by Culicoides had occurred. Cumulative collection and meteorological data suggest 15·6% of flights over 1 km were upwind of the treatment area and 84·4% downwind. Synthesis and applications. The use of immunomarking eliminates the potential adverse effects on survival and behaviour of insect collection prior to marking, substantially improving the resolution and accuracy of estimates of the dispersal potential of small and delicate vector species such as Culicoides. Using this technique, quantification of the range of Culicoides dispersal with regard to meteorological conditions including wind direction will enable improved, data‐driven modelling of the spread of Culicoides‐borne arboviruses and will inform policy response to incursions and outbreaks. The use of immunomarking eliminates the potential adverse effects on survival and behaviour of insect collection prior to marking, substantially improving the resolution and accuracy of estimates of the dispersal potential of small and delicate vector species such as Culicoides. Using this technique, quantification of the range of Culicoides dispersal with regard to meteorological conditions including wind direction will enable improved, data‐driven modelling of the spread of Culicoides‐borne arboviruses and will inform policy response to incursions and outbreaks.
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影响因子：
11.8
作者：
Elbers AR;Meiswinkel R;van Weezep E;Sloet van Oldruitenborgh-Oosterbaan MM;Kooi EA
通讯作者：
Kooi EA
影响因子：
4.9
作者：
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1.9
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.6
作者：
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通讯作者：
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