High-sucrose diet exposure is associated with selective and reversible alterations in the rat peripheral taste system.
High-sucrose diet exposure is associated with selective and reversible alterations in the rat peripheral taste system.
复制标题
高核饮食暴露与大鼠外围味觉系统的选择性和可逆变化有关。
DOI：
10.1016/j.cub.2022.07.063
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发表时间：
2022-10-10
期刊：
影响因子：
9.2
通讯作者：
Dus, Monica
中科院分区：
文献类型：
--
作者：
Sung, Hayeon;Vesela, Iva;Driks, Hannah;Ferrario, Carrie R.;Mistretta, Charlotte M.;Bradley, Robert M.;Dus, Monica
糖的摄入量增加与代谢疾病的风险增加有关。然而，来自人类、啮齿动物和昆虫的证据表明，膳食蔗糖会改变甜味感觉，但对外周神经或味蕾改变的了解很少。为了解决这一问题，雄性大鼠连续4周接触30%液体蔗糖（蔗糖大鼠）。然后将鼓索（CT）神经对糖、其他味觉、触摸和冷的舌刺激的神经生理反应与对照组（仅接触水）进行比较。对菌状乳头和味蕾进行了形态学和免疫组织化学分析。与对照组相比，蔗糖大鼠对0.15-2.0M蔗糖的CT反应显著降低。相比之下，葡萄糖，果糖，麦芽糖，糖精钠，氯化钠，有机酸或鲜味，触摸，或冷刺激没有观察到效果。而味蕾的数量，大小和神经支配体积不受影响，在菌状乳头中PLCβ2+味蕾细胞的数量减少。值得注意的是，用水代替蔗糖导致所有表型在4周内完全恢复。这项工作揭示了蔗糖消费对外周味觉神经反应和味蕾细胞的选择性和特定模式的影响，并对营养和代谢疾病风险产生影响。
Elevated sugar consumption is associated with an increased risk for metabolic diseases. Whereas evidence from humans, rodents, and insects suggests that dietary sucrose modifies sweet taste sensation, understanding of peripheral nerve or taste bud alterations is sparse. To address this, male rats were given access to 30% liquid sucrose for 4 weeks (Sucrose rats). Neurophysiological responses of the chorda tympani (CT) nerve to lingual stimulation with sugars, other taste qualities, touch, and cold were then compared to controls (access to water only). Morphological and immunohistochemical analyses of fungiform papillae and taste buds were also conducted. Sucrose rats had substantially decreased CT responses to 0.15–2.0M sucrose compared to controls. In contrast, effects were not observed for glucose, fructose, maltose, Na saccharin, NaCl, organic acid or umami, touch, or cold stimuli. Whereas taste bud number, size, and innervation volume were unaffected, the number of PLCβ2+ taste bud cells in the fungiform papilla was reduced in Sucrose rats. Notably, replacement of sucrose with water resulted in a complete recovery of all phenotypes over 4 weeks. The work reveals selective and modality-specific effects of sucrose consumption on peripheral taste nerve responses and taste bud cells, with implications for nutrition and metabolic disease risk.
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味觉的细胞生物学。
影响因子：
7.8
作者：
Chaudhari, Nirupa;Roper, Stephen D.
通讯作者：
Roper, Stephen D.
影响因子：
2.5
作者：
Clapp, TR;Yang, RB;Kinnamon, JC
通讯作者：
Kinnamon, JC
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3.1
作者：
Freeman, Clara R.;Zehra, Amna;Wang, Gene-Jack
通讯作者：
Wang, Gene-Jack
影响因子：
2.9
作者：
Cheron, Jean-Baptiste;Golebiowski, Jerome;Fiorucci, Sebastien
通讯作者：
Fiorucci, Sebastien
影响因子：
4.9
作者：
Kaufman, Andrew;Kim, Jennie;Dando, Robin
通讯作者：
Dando, Robin