Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation.
Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation.
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建立小鼠培养系统，用于模拟颅骨和躯干神经嵴细胞分化的时间进展。
DOI：
10.1242/dmm.035097
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发表时间：
2018-12-12
影响因子：
4.3
通讯作者：
Udvadia AJ
中科院分区：
文献类型：
--
作者：
Replogle MR;Sreevidya VS;Lee VM;Laiosa MD;Svoboda KR;Udvadia AJ
神经嵴（NC）是胚胎祖细胞的短暂群体，与多种先天性出生缺陷和儿科综合征有关。 NC 相关疾病的广泛谱可归因于 NC 产生的多种分化细胞类型。 NC 发育的体外模型提供了一个强大的平台，用于测试正常和致病条件下介导 NC 分化的内在和外在因素的相对贡献。尽管分化是一个随着时间的推移而展开的动态过程，但目前还没有明确的年表来表征 NC 分化向特定细胞命运的体外进展。在这项研究中，我们优化了原代小鼠 NC 细胞扩增的培养条件，即使在多次传代后，这些细胞也能产生外胚层和中外胚层衍生物。值得注意的是，我们已经描绘了高度可重复的时间表，其中包括体外谱系特异性 NC 分化的不同中间阶段。此外，从同一胚胎中分离出颅脑和躯干 NC 细胞，使我们能够在分化过程中对两个细胞群进行直接比较。我们的结果定义了细胞形态和行为的特征性变化，这些变化跟踪了 NC 细胞在体外沿神经元、神经胶质和软骨形成谱系分化时的时间进展。这些基准构成了一个按时间顺序排列的基线，用于评估遗传或环境破坏如何促进或阻碍 NC 分化。引入时间维度大大增强了该平台筛选药物或化学品的发育毒性或治疗潜力的能力。 摘要：一种分离和扩增原代神经嵴细胞并建立可重复的分化时间基准的新方法，为发育毒性或治疗能力提供了潜在的筛选平台。
The neural crest (NC) is a transient population of embryonic progenitors that are implicated in a diverse range of congenital birth defects and pediatric syndromes. The broad spectrum of NC-related disorders can be attributed to the wide variety of differentiated cell types arising from the NC. In vitro models of NC development provide a powerful platform for testing the relative contributions of intrinsic and extrinsic factors mediating NC differentiation under normal and pathogenic conditions. Although differentiation is a dynamic process that unfolds over time, currently, there is no well-defined chronology that characterizes the in vitro progression of NC differentiation towards specific cell fates. In this study, we have optimized culture conditions for expansion of primary murine NC cells that give rise to both ectodermal and mesoectodermal derivatives, even after multiple passages. Significantly, we have delineated highly reproducible timelines that include distinct intermediate stages for lineage-specific NC differentiation in vitro. In addition, isolating both cranial and trunk NC cells from the same embryos enabled us to make direct comparisons between the two cell populations over the course of differentiation. Our results define characteristic changes in cell morphology and behavior that track the temporal progression of NC cells as they differentiate along the neuronal, glial and chondrogenic lineages in vitro. These benchmarks constitute a chronological baseline for assessing how genetic or environmental disruptions may facilitate or impede NC differentiation. Introducing a temporal dimension substantially increases the power of this platform for screening drugs or chemicals for developmental toxicity or therapeutic potential. Summary: A novel method for isolating and expanding primary neural crest cells, and establishment of reproducible temporal benchmarks of differentiation, provides a potential screening platform for developmental toxicity or therapeutic capacity.
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DOI：
10.1242/dev.002642
发表时间：
2007-06
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Billon N;Iannarelli P;Monteiro MC;Glavieux-Pardanaud C;Richardson WD;Kessaris N;Dani C;Dupin E
通讯作者：
Dupin E
DOI：
10.1002/dvg.23105
发表时间：
2018-06
期刊：
Genesis (New York, N.Y. : 2000)
影响因子：
--
作者：
Debbache J;Parfejevs V;Sommer L
通讯作者：
Sommer L
DOI：
10.1073/pnas.0504750102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Akiyama, H;Kim, JE;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
2.7
作者：
COHEN, AM;KONIGSBERG, IR
通讯作者：
KONIGSBERG, IR
DOI：
10.1007/bf01148594
发表时间：
1984-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
JESSEN, KR;MIRSKY, R
通讯作者：
MIRSKY, R