Evidence of long distance airborne transport of porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae.
Evidence of long distance airborne transport of porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae.
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DOI：
10.1051/vetres/2009022
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发表时间：
2009-07
影响因子：
4.4
通讯作者：
Deen J
中科院分区：
文献类型：
--
作者：
Dee S;Otake S;Oliveira S;Deen J
评价了猪繁殖与呼吸综合征病毒（PRRSV）和猪肺炎支原体（Mycoplasma hyoprophylaxis）通过空气传播途径进行长距离传播的能力。用猪繁殖与呼吸综合征病毒MN-184和M.在50天的时间内，使用液体旋风收集器在距源畜群指定距离处收集空气样本。检测样品中是否存在PRRSV RNA和M。通过PCR检测猪肺炎支原体DNA，如果阳性，则进一步表征。在306份样品中，4份（1.3%）为PRRSV RNA阳性，6份（1.9%）为M阳性。猪舌DNA。PRRSV阳性样本被回收到4.7公里的西北（NW）的源人口。四是M。在西北采样点获得了猪肺炎支原体阳性样本; 2个样本在距离源种群约2.3公里处，另外2个样本在距离源种群约4.7公里处。在其余2个样品中，一个样品在东南采样点获得，另一个在西南采样点获得，两个位置距离源约4.7公里。4份PRRSV阳性样本含有感染性病毒，与用于鉴定来源人群的MN-184分离株同源性≥ 98.8%。所有6个M。猪肺炎支原体阳性标本与M. 232. history这些结果支持了这一假设，即这些重要的猪病原体可以发生长距离空气传播。
The ability of porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae to be transported over long distances via the airborne route was evaluated. A source population of 300 grow-finish pigs was experimentally inoculated with PRRSV MN-184 and M. hyopneumoniae 232 and over a 50-day period, air samples were collected at designated distances from the source herd using a liquid cyclonic collector. Samples were tested for the presence of PRRSV RNA and M. hyopneumoniae DNA by PCR and if positive, further characterized. Of the 306 samples collected, 4 (1.3%) were positive for PRRSV RNA and 6 (1.9%) were positive for M. hyopneumoniae DNA. The PRRSV-positive samples were recovered 4.7 km to the northwest (NW) of the source population. Four of the M. hyopneumoniae-positive samples were obtained at the NW sampling point; 2 samples at approximately 2.3 km and the other 2 samples approximately 4.7 km from the source population. Of the remaining 2 samples, one sample was obtained at the southeast sampling point and the other at the southwest sampling point, with both locations being approximately 4.7 km from the source. The four PRRSV-positive samples contained infectious virus and were ≥ 98.8% homologous to the MN-184 isolate used to inoculate the source population. All 6 of the M. hyopneumoniae-positive samples were 99.9% homologous to M. hyopneumoniae 232. These results support the hypothesis that long distance airborne transport of these important swine pathogens can occur.
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