Examination of host genetic effects on nasal microbiome composition.
Examination of host genetic effects on nasal microbiome composition.
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DOI：
10.1016/j.jaci.2022.06.004
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发表时间：
2022-11
影响因子：
14.2
通讯作者：
Bunyavanich, Supinda
中科院分区：
文献类型：
--
作者：
Irizar, Haritz;Chun, Yoojin;Arditi, Zoe;Do, Anh;Grishina, Galina;Grishin, Alexander;Vicencio, Alfin;Bunyavanich, Supinda
遗传易感性会增加哮喘的风险，并且不同的鼻微生物组成与哮喘有关。宿主遗传学可能会影响鼻腔微生物组的组成。我们研究了宿主遗传学与鼻微生物组组成之间的关联。从美国纽约西奈山卫生系统的584名参与者中收集鼻样品。从40名参与者的子集中收集了77份随访样本。对鼻样本进行16 S rRNA测序和RNA测序。计算β-多样性，对RNA-seq数据进行变体调用，并确定个体之间的遗传相关性。使用线性回归模型，我们测试了遗传相关性与鼻微生物组组成之间的关联。队列的中位年龄为14.6岁（IQR 11.2-19.5），参与者代表不同的祖先，52.7%的队列女性。对于提供随访样本的参与者，样本之间的中位时间为5.1个月（IQR 1.4-7.2）。随着时间的推移，受试者体内的β多样性所反映的鼻微生物组组成相似性显著高于受试者之间（系数=0.091，p值=2.84−07）。遗传相关性和β多样性之间没有显著关联（系数=-0.05，p值=0.29）。探索β多样性和遗传方差之间关系的其他分析也得到了类似的结果。我们的研究结果表明，宿主遗传学对鼻腔微生物组组成的影响很小。这项宿主遗传学和气道微生物组组成的研究显示，随着时间的推移，受试者体内的鼻微生物组组成的相似性高于受试者之间的相似性，并且宿主遗传学在塑造鼻微生物组组成方面几乎没有作用。
Genetic predisposition increases risk for asthma, and distinct nasal microbial compositions are associated with asthma. Host genetics might shape nasal microbiome composition. We examined associations between host genetics and nasal microbiome composition. Nasal samples were collected from 584 participants from the Mount Sinai Health System, New York, USA. Seventy-seven follow-up samples were collected from a subset of forty participants. 16S rRNA sequencing and RNA-sequencing were performed on nasal samples. Beta-diversity was calculated, variant calling on RNA-seq data was performed, and genetic relatedness between individuals was determined. Using linear regression models, we tested for associations between genetic relatedness and nasal microbiome composition. The median age of the cohort was 14.6 years (IQR 11.2–19.5), with participants representing diverse ancestries and 52.7% of the cohort female. For participants who provided follow-up samples, the median time between samples was 5.1 months (IQR 1.4–7.2). Nasal microbiome composition similarity as reflected by beta diversity was significantly higher within subjects over time vs. between subjects (coefficient=0.091, p-value=2.84−07). There was no significant association between genetic relatedness and beta diversity (coefficient=−0.05, p-value=0.29). Additional analyses exploring the relationship between beta-diversity and genetic variance yielded similar results. Our results suggest that host genetics has little influence on nasal microbiome composition. This study of host genetics and airway microbiome composition revealed higher similarity in nasal microbiome composition within subject over time than between subjects, and that host genetics plays little role in shaping nasal microbiome composition.
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DOI：
10.1073/pnas.1205624109
发表时间：
2012-05-22
影响因子：
11.1
作者：
Hanski, Ilkka;von Hertzen, Leena;Haahtela, Tari
通讯作者：
Haahtela, Tari
DOI：
10.1016/j.jaci.2018.02.020
发表时间：
2018-09
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Fazlollahi M;Lee TD;Andrade J;Oguntuyo K;Chun Y;Grishina G;Grishin A;Bunyavanich S
通讯作者：
Bunyavanich S
DOI：
10.1016/j.jaci.2021.01.031
发表时间：
2021-07
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Chun Y;Do A;Grishina G;Arditi Z;Ribeiro V;Grishin A;Vicencio A;Bunyavanich S
通讯作者：
Bunyavanich S
影响因子：
16.6
作者：
van Dongen J;Nivard MG;Willemsen G;Hottenga JJ;Helmer Q;Dolan CV;Ehli EA;Davies GE;van Iterson M;Breeze CE;Beck S;BIOS Consortium;Suchiman HE;Jansen R;van Meurs JB;Heijmans BT;Slagboom PE;Boomsma DI
通讯作者：
Boomsma DI
影响因子：
64.8
作者：
Rothschild, Daphna;Weissbrod, Omer;Segal, Eran
通讯作者：
Segal, Eran