Sodium hydrosulfide induces systemic thermotolerance to strawberry plants through transcriptional regulation of heat shock proteins and aquaporin.
Sodium hydrosulfide induces systemic thermotolerance to strawberry plants through transcriptional regulation of heat shock proteins and aquaporin.
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DOI：
10.1186/1471-2229-14-42
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发表时间：
2014-02-05
影响因子：
5.3
通讯作者：
Fotopoulos V
中科院分区：
文献类型：
--
作者：
Christou A;Filippou P;Manganaris GA;Fotopoulos V
极端温度是植物生长和生产力的重要限制因素。本研究评价了水培预处理对草莓(Fragaria x ananassa cv。以H2S供体氢硫化钠（NaHS, 100 μM, 48 h）处理Camarosa （Camarosa）根，研究植物对急性热休克处理（42°C, 8 h）的反应。在nahs预处理的植株中，热胁迫诱导的表型损伤得到改善，与胁迫植株相比，nahs预处理植株保持了更高的最大光化学PSII量子产量。由于丙二醛（MDA）、H2O2和一氧化氮（NO）含量低于热胁迫植物，H2S预处理对氧化和亚硝化二次胁迫有明显的缓解作用。此外，NaHS预处理可以保持抗坏血酸/谷胱甘肽的稳态，在热胁迫8小时后，ASC和GSH池氧化还原干扰降低，ASC （GDH）和GSH生物合成酶（GS， GCS）的转录增强。此外，NaHS根预处理还诱导了一系列保护性分子的基因表达水平，如酶促抗氧化剂（cAPX、CAT、MnSOD、GR）、热休克蛋白（HSP70、HSP80、HSP90）和水通道蛋白（PIP）。总之，我们认为H2S预处理在转录水平上激活了热休克防御相关通路的协调网络，并系统地保护草莓植株免受热休克诱导的损伤。
Temperature extremes represent an important limiting factor to plant growth and productivity. The present study evaluated the effect of hydroponic pretreatment of strawberry (Fragaria x ananassa cv. ‘Camarosa’) roots with an H2S donor, sodium hydrosulfide (NaHS; 100 μM for 48 h), on the response of plants to acute heat shock treatment (42°C, 8 h). Heat stress-induced phenotypic damage was ameliorated in NaHS-pretreated plants, which managed to preserve higher maximum photochemical PSII quantum yields than stressed plants. Apparent mitigating effects of H2S pretreatment were registered regarding oxidative and nitrosative secondary stress, since malondialdehyde (MDA), H2O2 and nitric oxide (NO) were quantified in lower amounts than in heat-stressed plants. In addition, NaHS pretreatment preserved ascorbate/glutathione homeostasis, as evidenced by lower ASC and GSH pool redox disturbances and enhanced transcription of ASC (GDH) and GSH biosynthetic enzymes (GS, GCS), 8 h after heat stress imposition. Furthermore, NaHS root pretreatment resulted in induction of gene expression levels of an array of protective molecules, such as enzymatic antioxidants (cAPX, CAT, MnSOD, GR), heat shock proteins (HSP70, HSP80, HSP90) and aquaporins (PIP). Overall, we propose that H2S root pretreatment activates a coordinated network of heat shock defense-related pathways at a transcriptional level and systemically protects strawberry plants from heat shock-induced damage.
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影响因子：
2.6
作者：
Kumar, Sanjeev;Kaur, Ramanpreet;Nayyar, Harsh
通讯作者：
Nayyar, Harsh
影响因子：
9.4
作者：
Garcia-Mata, Carlos;Lamattina, Lorenzo
通讯作者：
Lamattina, Lorenzo
DOI：
10.1073/pnas.95.7.3513
发表时间：
1998-03-31
影响因子：
11.1
作者：
Horváth, I;Glatz, A;Vígh, L
通讯作者：
Vígh, L
影响因子：
3.7
作者：
Allakhverdiev, Suleyman I.;Murata, Norio
通讯作者：
Murata, Norio
影响因子：
6.5
作者：
Ayadi, Malika;Cavez, Damien;Masmoudi, Khaled
通讯作者：
Masmoudi, Khaled