Improved spiral tube assembly for high-speed counter-current chromatography.
Improved spiral tube assembly for high-speed counter-current chromatography.
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DOI：
10.1016/j.chroma.2008.10.126
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发表时间：
2009-05-08
期刊：
影响因子：
--
通讯作者：
Finn TM
中科院分区：
文献类型：
--
作者：
Ito Y;Clary R;Powell J;Knight M;Finn TM
原来的螺旋管支撑（STS）组件通过改变管子的形状进行了改进，沿长度方向垂直施压 1 厘米。这种修改中断了流动相的层流。到转子中心的4个回流槽中的管路通过特制的压具压平，以减少死体积，从而提高柱效率。该螺旋管组件的性能在使用合适的极性两相溶剂系统分离二肽和蛋白质时进行了测试。结果表明，本系统在较短的洗脱时间内产生高分配效率和令人满意的固定相保留水平。本发明的高速逆流色谱系统将有效地应用于广泛的两相溶剂系统，包括用于分离生物聚合物如蛋白质和核酸的水-水聚合物相系统。 。
The original spiral tube support (STS) assembly is improved by changing the shape of the tubing, with 1-cm presses perpendicularly along the length. This modification interrupts the laminar flow of the mobile phase. The tubing in the 4 return grooves to the center of the rotor is flattened by a specially made pressing tool to decrease the dead volume and thus increase the column efficiency. The performance of this spiral tube assembly was tested in separations of dipeptides and proteins with suitable polar two-phase solvent systems. The results revealed that the present system yields high partition efficiency with a satisfactory level of stationary phase retention in a short elution time. The present high-speed counter-current chromatographic system will be efficiently applied to a broad spectrum of two-phase solvent systems including aqueous-aqueous polymer phase systems which are used for separation of biopolymers such as proteins and nucleic acids. .
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DOI：
10.1080/10826070802019913
发表时间：
2008-01-01
影响因子：
1.3
作者：
Ito, Y.;Clary, R.;Finn, T. M.
通讯作者：
Finn, T. M.
影响因子：
4.1
作者：
Ito, Yoichiro;Qi, Lin;Li, Ting
通讯作者：
Li, Ting
DOI：
10.1081/jlc-120021255
发表时间：
2003-01-01
影响因子：
1.3
作者：
Ito, Y;Yang, FQ;Sullivan, JV
通讯作者：
Sullivan, JV
影响因子：
4.1
作者：
Ito, Yoichiro;Clary, Robert;Powell, Jimmie
通讯作者：
Powell, Jimmie