Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease.
Pathways to clinical CLARITY: volumetric analysis of irregular, soft, and heterogeneous tissues in development and disease.
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DOI：
10.1038/s41598-017-05614-4
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发表时间：
2017-07-19
影响因子：
4.6
通讯作者：
Deisseroth K
中科院分区：
文献类型：
--
作者：
Hsueh B;Burns VM;Pauerstein P;Holzem K;Ye L;Engberg K;Wang AC;Gu X;Chakravarthy H;Arda HE;Charville G;Vogel H;Efimov IR;Kim S;Deisseroth K
典型的薄片组织学不能很好地捕捉三维组织结构关系，这给组织生理学和病理学的研究带来了挑战。此外，虽然最近的进展已经取得了完整的方法，用于清除，标记和成像整个器官，如成熟的大脑，这些方法通常是不适合的软，不规则，和异质组织占绝大多数的临床样品和活检。在这里，我们开发了一种双相水凝胶方法，该方法沿着自动化分析，提供了对空间不规则和易碎组织结构的高通量定量体积询问。我们验证和应用这种方法在各种发展和患病组织的检查，特别关注正常和病理胰腺神经支配和发展的动态，包括在临床样本。与传统的薄片组织学相比，完整组织方法的定量优势得到了证明，在研究和临床环境中具有广泛的应用。
Three-dimensional tissue-structural relationships are not well captured by typical thin-section histology, posing challenges for the study of tissue physiology and pathology. Moreover, while recent progress has been made with intact methods for clearing, labeling, and imaging whole organs such as the mature brain, these approaches are generally unsuitable for soft, irregular, and heterogeneous tissues that account for the vast majority of clinical samples and biopsies. Here we develop a biphasic hydrogel methodology, which along with automated analysis, provides for high-throughput quantitative volumetric interrogation of spatially-irregular and friable tissue structures. We validate and apply this approach in the examination of a variety of developing and diseased tissues, with specific focus on the dynamics of normal and pathological pancreatic innervation and development, including in clinical samples. Quantitative advantages of the intact-tissue approach were demonstrated compared to conventional thin-section histology, pointing to broad applications in both research and clinical settings.
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影响因子：
8.8
作者：
Borden P;Houtz J;Leach SD;Kuruvilla R
通讯作者：
Kuruvilla R
影响因子：
2.2
作者：
Kim A;Miller K;Jo J;Kilimnik G;Wojcik P;Hara M
通讯作者：
Hara M
影响因子：
3.6
作者：
DONEV, SR
通讯作者：
DONEV, SR
DOI：
10.1152/ajpendo.2001.280.2.e378
发表时间：
2001-02-01
影响因子：
5.1
作者：
Benthem, L;Mundinger, TO;Taborsky, GJ
通讯作者：
Taborsky, GJ
影响因子：
7.4
作者：
Groene, Eva;Ueceyler, Nurcan;Lang, Philip M.
通讯作者：
Lang, Philip M.