Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.

Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
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DOI:
10.1371/journal.pone.0046857
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发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Jaeger KE
Jaeger KE
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Funken H;Bartels KM;Wilhelm S;Brocker M;Bott M;Bains M;Hancock RE;Rosenau F;Jaeger KE

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岩藻糖结合凝集素LecB影响铜绿假单胞菌生物被膜的形成并参与其致病性。LecB存在于外膜中,并且可以通过用岩藻糖处理外膜级分而特异性地释放,这表明其与特异性配体结合。在这里,我们报告说,LecB结合外膜蛋白OprF。在OprF缺陷的铜绿假单胞菌突变体中,LecB不再在膜中检测到,而是在培养物上清液中检测到,表明LecB和OprF之间的特异性相互作用。
The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.
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