A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
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一种同步测量茶树不同叶面角质层蒸腾作用的方法
DOI：
10.3389/fpls.2020.00420
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发表时间：
2020-05
影响因子：
5.6
通讯作者：
Mingjie Chen
中科院分区：
文献类型：
--
作者：
Yi Zhang;Xiaobing Chen;Zhenghua Du;Wenjing Zhang;Ananta Raj Devkota;Zijian Chen;Changsong Chen;Weijiang Sun;Mingjie Chen
植物角质层是限制水分无限制流失的主要屏障，因此在植物耐旱性中起着关键作用。由于叶片远轴面气孔的存在，测量远轴角质层蒸腾在技术上具有挑战性。现有的研究多集中在叶片近轴面气孔的研究上，而远轴面角质层蒸腾的研究较少。发展一种能够同时测量两个叶片表面角质层蒸腾的方法，将有助于提高我们对叶片蒸腾屏障组织的理解。在这里，我们开发了一种新的方法，使同时测量角质层蒸腾速率从近轴和远轴表面。所提出的方法结合了多步叶预处理，包括在黑暗下的水平衡和阿坝处理，以关闭气孔，以及阿拉伯树胶或凡士林的应用，以去除或密封的表皮蜡层。建立了由实测数据计算叶片表面蒸腾速率的数学公式。这种方法有利于同时定量角质蒸腾从近轴和远轴叶表面。应用这种方法，我们证明了近轴的角质层内蜡质和远轴的角质层外蜡质构成了茶树的主要蒸腾障碍。蜡分析表明，近轴面内蜡具有较高的覆盖率的非常长的链脂肪酸，1-烷醇酯，和乙二醇，这可能是由于其较高的蒸腾屏障比远轴面内蜡。
The plant cuticle is the major barrier that limits unrestricted water loss and hence plays a critical role in plant drought tolerance. Due to the presence of stomata on the leaf abaxial surface, it is technically challenging to measure abaxial cuticular transpiration. Most of the existing reports were only focused on leaf astomatous adaxial surface, and few data are available regarding abaxial cuticular transpiration. Developing a method that can measure cuticular transpiration from both leaf surfaces simultaneously will improve our understanding about leaf transpiration barrier organization. Here, we developed a new method that enabled the simultaneous measurement of cuticular transpiration rates from the adaxial and abaxial surfaces. The proposed method combined multi-step leaf pretreatments including water equilibration under dark and ABA treatment to close stomata, as well as gum arabic or vaseline application to remove or seal the epicuticular wax layer. Mathematical formulas were established and used to calculate the transpiration rates of individual leaf surfaces from observed experimental data. This method facilitates the simultaneous quantification of cuticular transpiration from adaxial and abaxial leaf surfaces. By applying this method, we demonstrated that the adaxial intracuticular waxes and the abaxial epicuticular waxes constitute the major transpiration barriers in Camellia sinensis. Wax analysis indicated that adaxial intracuticular waxes had higher coverage of very long chain fatty acids, 1-alkanol esters, and glycols, which may be attributed to its higher transpiration barrier than that of the abaxial intracuticular waxes.
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影响因子：
6.4
作者：
P. Holmgren;Paul G. Jarvis;Margaret S. Jarvis
通讯作者：
P. Holmgren;Paul G. Jarvis;Margaret S. Jarvis
影响因子：
7.4
作者：
JENKS, MA;JOLY, RJ;ASHWORTH, EN
通讯作者：
ASHWORTH, EN
影响因子：
20.5
作者：
Kerstiens, G
通讯作者：
Kerstiens, G
影响因子：
11.6
作者：
Sieber, P;Schorderet, M;Nawrath, C
通讯作者：
Nawrath, C
影响因子：
5.6
作者：
Guzmán P;Fernández V;Graça J;Cabral V;Kayali N;Khayet M;Gil L
通讯作者：
Gil L