Moderate systemic therapeutic hypothermia is insufficient to protect blood-spinal cord barrier in spinal cord injury.
Moderate systemic therapeutic hypothermia is insufficient to protect blood-spinal cord barrier in spinal cord injury.
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DOI：
10.3389/fneur.2022.1041099
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发表时间：
2022
影响因子：
3.4
通讯作者：
中科院分区：
文献类型：
--
作者：
血-脊髓屏障（BSCB）破坏是脊髓损伤（SCI）的关键事件，可加重继发性损伤，但无特异性治疗。以往的研究表明，全身治疗性低温（TH）可以保护脑损伤后的血脑屏障。为了验证SCI后BSCB上是否存在类似的作用，在32°C下在具有挫伤-SCI的小鼠上诱导中度全身性TH持续4小时。在体双光子显微镜动态观察脊髓损伤后1 h BSCB渗漏情况，并结合免疫组化法检测脊髓损伤后1 h和4 h BSCB渗漏情况。BSCB渗漏在常温（NT）和TH组之间在体内和死后水平上没有差异。内皮细胞紧密连接的表达无显着差异，NT和TH组SCI后4小时，检测毛细管蛋白质印迹。脊髓损伤后4 h TH对BSCB的结构损伤无明显影响，但对连接间隙的形成无明显影响。我们的研究结果表明，中度全身TH诱导4小时不具有保护作用的破坏BSCB在早期SCI。这种治疗方法价值低，不推荐用于早期SCI的BSCB中断治疗。
Blood–spinal cord barrier (BSCB) disruption is a pivotal event in spinal cord injury (SCI) that aggravates secondary injury but has no specific treatment. Previous reports have shown that systemic therapeutic hypothermia (TH) can protect the blood–brain barrier after brain injury. To verify whether a similar effect exists on the BSCB after SCI, moderate systemic TH at 32°C was induced for 4 h on the mice with contusion-SCI. In vivo two-photon microscopy was utilized to dynamically monitor the BSCB leakage 1 h after SCI, combined with immunohistochemistry to detect BSCB leakage at 1 and 4 h after SCI. The BSCB leakage was not different between the normothermia (NT) and TH groups at both the in vivo and postmortem levels. The expression of endothelial tight junctions was not significantly different between the NT and TH groups 4 h after SCI, as detected by capillary western blotting. The structural damage of the BSCB was examined with immunofluorescence, but the occurrence of junctional gaps was not changed by TH 4 h after SCI. Our results have shown that moderate systemic TH induced for 4 h does not have a protective effect on the disrupted BSCB in early SCI. This treatment method has a low value and is not recommended for BSCB disruption therapy in early SCI.
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