Blastomeres derived from the vegetal pole provide extra-embryonic nutrition to sturgeon (Acipenser) embryos: Transition from holoblastic to meroblastic cleavage
Blastomeres derived from the vegetal pole provide extra-embryonic nutrition to sturgeon (Acipenser) embryos: Transition from holoblastic to meroblastic cleavage
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来自植物极的卵裂球为鲟鱼胚胎提供胚胎外营养：从全胚细胞分裂到粒细胞分裂的转变
DOI：
10.1016/j.aquaculture.2022.737899
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发表时间：
2022
期刊：
影响因子：
4.5
通讯作者：
Saito Taiju
中科院分区：
文献类型：
--
作者：
Shah Mujahid Ali;Fatira Effrosyni;Iegorova Viktoriia;Xie Xuan;Gela David;Rodina Marek;Fran?k Roman;P?eni?ka Martin;Saito Taiju
一般来说，胚胎中的全胚卵裂（如两栖动物），其中所有的卵裂球都贡献给一个胚层，被保存为脊椎动物的干谱系，而部分卵裂在每个脊椎动物谱系中独立进化。卵的大小：卵黄体积的增加是全裂型向部分裂型转变的关键因素。鲟鱼的卵比非洲爪蟾的卵大两倍;尽管大小不同，鲟鱼胚胎保留了几乎相同的发育特征。光滑。相比之下，来自鲟鱼胚胎的植物极（VP）的卵裂球的命运是不明的。因此，本研究的目的是确定胚胎的VP是否有助于胚胎发育，或者仅仅是胚胎外的。这也可能揭示是否在actinopterygian谱系的卵裂模式（全胚层到部分卵裂）的过渡与鸡蛋的大小：蛋黄体积。在这里，我们发现鲟鱼植物性卵裂球仅形成原始生殖细胞，其余部分由细胞卵黄（卵黄细胞; YCs）组成。形态学和表型特征显示，1 k-细胞/中期囊胚过渡后，YCs变得转录不活跃，只为幼虫提供营养，因为它们的发展。此外，植物卵裂球的抑制表明，鲟鱼可以利用他们的蛋黄在一个无细胞的形式，类似硬骨鱼，这意味着在放线菌，硬骨鱼，部分卵裂可能已经进化的植物卵裂球的融合。
Generally, holoblastic cleavage in embryos (as in amphibians) in which all blastomeres contribute to one of the germ layers, are preserved as a stem lineage of vertebrates, and meroblastic cleavage has evolved independently in each vertebrate lineage. The increasing egg size: yolk volume is the key factor for transition from holoblastic to meroblastic cleavage patterns. Sturgeon (Acipenser) eggs are two times larger than those of the African clawed frogXenopus laevis(amphibian); despite the varying size, sturgeon embryos retain nearly the same developmental characteristics asX. laevis. Comparatively, the fate of blastomeres derived from the vegetal pole (VP) of a sturgeon embryo is unspecified. Thus, the goal of this study was to determine whether the VP of the embryo contributes to embryonic development, or is simply extra-embryonic. This may also reveal whether the transition of the cleavage pattern (holoblastic to meroblastic) in the actinopterygian lineage is correlated with the egg size: yolk volume. Here, we found that sturgeon vegetal blastomeres formed only primordial germ cells, and the rest were made up of cellular yolk (yolk cells; YCs). Morphological and phenotypic characteristics revealed that after the 1 k-cell / mid-blastula transition, YCs became transcriptionally inactive and served only to provide nutrition to larvae as they developed. Furthermore, inhibition of vegetal blastomeres revealed that sturgeon can utilize their yolk in an acellular form, similar to teleosts, implying that meroblastic cleavage in the actinopterygians, like teleosts, might have evolved by the fusion of the vegetal blastomeres.
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影响因子：
7.3
作者：
Fu L;Shi YB
通讯作者：
Shi YB
影响因子：
0.7
作者：
Saito, Taiju;Fujimoto, Takafumi;Yamaha, Etsuro
通讯作者：
Yamaha, Etsuro
黑氏鲟和非洲爪蛙非洲爪蟾卵母细胞 mRNA 定位模式的比较
DOI：
10.1002/jez.b.22802
发表时间：
2018
期刊：
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
影响因子：
--
作者：
K. Pocherniaieva;Monika Šídová;M. Havelka;Taiju Saito;M. Pšenička;Radek Sindelka;V. Kašpar
通讯作者：
V. Kašpar
硬骨鱼类原肠胚形成的系统发育视角
影响因子：
2.9
作者：
A. Collazo;J. Bolker;R. Keller
通讯作者：
R. Keller
影响因子：
2.5
作者：
Buchholz, Daniel R.;Singamsetty, Srikanth;Elinson, Richard P.
通讯作者：
Elinson, Richard P.