Aligned electrospun scaffolds and elastogenic factors for vascular cell-mediated elastic matrix assembly.
Aligned electrospun scaffolds and elastogenic factors for vascular cell-mediated elastic matrix assembly.
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DOI：
10.1002/term.470
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发表时间：
2012-10
影响因子：
3.3
通讯作者：
Ramamurthi, Anand
中科院分区：
文献类型：
--
作者：
Bashur, Chris A.;Ramamurthi, Anand
关键词：
促进平滑肌细胞（SMCs）产生有组织弹性基质的策略在工程功能血管中是至关重要的。因此，本研究的目的是确定不同表面（即随机和排列的静电纺聚（ε-己内酯）网和二维对照）和外源弹性因子对培养大鼠主动脉SMC表型和细胞外基质产生的影响。这项研究表明，排列的电纺丝纤维引导细胞排列，诱导更细长的细胞形态，并促进更合成的表型。重要的是，这些细胞在电纺丝支架上每个细胞产生更多的富弹性蛋白基质。此外，外源性弹性因子严重限制了RASMC的增殖，并在静电纺网上促进了更合成的SMC表型，但它们对二维对照的影响较小。最后，弹性因子诱导SMCs在每个细胞的基础上产生更多的基质胶原和弹性蛋白。总之，这些结果证明了静电纺网的弹性优势。
Strategies to enhance the production of organized elastic matrix by smooth muscle cells (SMCs) are critical in engineering functional vascular conduits. Therefore, the goal of this study was to determine the effect of different surfaces (i.e. random and aligned electrospun poly(ε-caprolactone) meshes and two-dimensional controls) and exogenous elastogenic factors on cultured rat aortic SMC phenotype and production of extracellular matrix. This study demonstrated that aligned electrospun fibers guide cell alignment, induce a more elongated cell morphology, and promote a more synthetic phenotype. Importantly, these cells produced greater amounts of elastin-rich matrix per cell on the electrospun scaffolds. In addition, exogenous elastogenic factors severely limited RASMC proliferation and promoted a more synthetic SMC phenotype on electrospun meshes, but they had less effect on two-dimensional controls. Finally, the elastogenic factors induced the SMCs to generate more matrix collagen and elastin on a per cell basis. Together, these results demonstrate the elastogenic benefits of electrospun meshes.
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