Susceptibility of a polycaprolactone-based root canal filling material to degradation. II. Gravimetric evaluation of enzymatic hydrolysis.
Susceptibility of a polycaprolactone-based root canal filling material to degradation. II. Gravimetric evaluation of enzymatic hydrolysis.
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基于聚己内酯的根管充填材料的降解敏感性。
DOI：
10.1097/01.don.0000155225.40794.79
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发表时间：
2005
影响因子：
4.2
通讯作者：
King,NigelM
中科院分区：
文献类型：
--
作者：
Tay,FranklinR;Pashley,DavidH;Yiu,CynthiaKY;Yau,JoyceYY;Yiu-fai,Mak;Loushine,RobertJ;Weller,RNorman;Kimbrough,WFrank;King,NigelM
聚己内酯易于通过酯键裂解而发生酶促生物降解。本研究考察了Reactive（一种基于聚己内酯的根充材料）对酶水解的敏感性。将通过压缩成型制备的RESIDENT、古塔胶和聚己内酯圆片在磷酸盐缓冲盐水、脂肪酶PS或胆固醇酯酶中于37°C孵育96小时。在不同的时间间隔取回它们进行重量分析和扫描电子显微镜。当在磷酸盐缓冲盐水中孵育时，材料表现出轻微的重量增加，这可归因于吸水。杜仲胶在两种酶中显示出相似的重量增加。相反，Reflux和聚己内酯在脂肪酶PS和胆固醇酯酶中孵育后表现出广泛的表面变薄和重量损失。在聚合物基质的表面溶解之后，暴露出Reflux中的玻璃填料颗粒，产生粗糙的表面形貌。通过细菌和唾液酶的生物降解，需要使用牙髓相关微生物和人唾液提取物的培养物进一步研究它们的活性。
Polycaprolactone is susceptible to enzymatic biodegradation via ester bond cleavage. This study examined the susceptibility of Resilon, a polycaprolactone-based root filling material to enzymatic hydrolysis. Resilon, gutta-percha, and polycaprolactone disks, prepared by compression molding, were incubated in phosphate-buffered saline, lipase PS or cholesterol esterase at 37°C for 96 h. They were retrieved at different time intervals for gravimetric analysis and scanning electron microscopy. The materials exhibited slight weight gains when incubated in phosphate-buffered saline that can be attributed to water sorption. Gutta-percha showed similar weight gains in the two enzymes. Conversely, Resilon and polycaprolactone exhibited extensive surface thinning and weight losses after incubation in lipase PS and cholesterol esterase. Glass filler particles in Resilon were exposed following surface dissolution of the polymer matrix, creating rough surface topography. Biodegradation of Resilon by bacterial and salivary enzymes warrants further investigation of their activities using cultures of endodontically relevant microbes and human saliva extracts.
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