Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi.
Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi.
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DOI：
10.1371/journal.pone.0165724
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Ritchie MG
中科院分区：
文献类型：
--
作者：
Vigoder FM;Parker DJ;Cook N;Tournière O;Sneddon T;Ritchie MG
冷适应是应对季节性寒冷的关键生理适应。通过提高耐寒能力，个体可以在冬季开始时保持更长时间的活动，并且可以更快地从冷休克中恢复。在昆虫中，尽管有许多生理学研究，但人们对冷适应的遗传基础知之甚少。最近，果蝇 virilis 和 D. montana 的转录组分析通过鉴定暴露于寒冷期间上调的基因，揭示了冷适应的候选基因。在这里，我们使用 RNAi 方法测试了肌醇 1-磷酸合酶 (Inos) 在蒙大拿州石竹耐冷性中的作用。蒙大拿D. montana环极地分布，成虫在极寒的北纬地区越冬。我们使用两个指标评估了 dsRNA 敲低果蝇的冷耐受性：冷昏迷恢复时间 (CCRT) 和冷驯化后的死亡率。注射 dsRNAInos 不会改变 CCRT，无论是总体上还是与冷处理相互作用，但它确实诱导了冷特异性死亡率，在注射的果蝇中观察到在 5°C 环境下适应但在 19°C 环境下没有观察到高死亡率。总体而言，注射 dsRNAInos 导致这种通常耐冷的物种的温度敏感性死亡率超过 60%。 qPCR 分析证实 dsRNA 注射成功降低了 Inos 的基因表达。因此，我们的结果表明 Inos 参与提高蒙大拿州石竹的耐冷性。还讨论了 Inos 提高耐冷性的潜在机制。
Cold acclimation is a critical physiological adaptation for coping with seasonal cold. By increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. In insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic analyses in Drosophila virilis and D. montana revealed candidate genes for cold acclimation by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate synthase (Inos), in cold tolerance in D. montana using an RNAi approach. D. montana has a circumpolar distribution and overwinters as an adult in northern latitudes with extreme cold. We assessed cold tolerance of dsRNA knock-down flies using two metrics: chill-coma recovery time (CCRT) and mortality rate after cold acclimation. Injection of dsRNAInos did not alter CCRT, either overall or in interaction with the cold treatment, however it did induced cold-specific mortality, with high levels of mortality observed in injected flies acclimated at 5°C but not at 19°C. Overall, injection with dsRNAInos induced a temperature-sensitive mortality rate of over 60% in this normally cold-tolerant species. qPCR analysis confirmed that dsRNA injection successfully reduced gene expression of Inos. Thus, our results demonstrate the involvement of Inos in increasing cold tolerance in D. montana. The potential mechanisms involved by which Inos increases cold tolerance are also discussed.
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影响因子：
3.4
作者：
Henry SA;Gaspar ML;Jesch SA
通讯作者：
Jesch SA
DOI：
10.1073/pnas.1405500111
发表时间：
2014-07-22
影响因子：
11.1
作者：
Port, Fillip;Chen, Hui-Min;Bullock, Simon L.
通讯作者：
Bullock, Simon L.
DOI：
10.1073/pnas.0708074105
发表时间：
2008-01-08
影响因子：
11.1
作者：
Kristensen, Torsten N.;Hoffmann, Ary A.;Loeschcke, Volker
通讯作者：
Loeschcke, Volker
影响因子：
2.9
作者：
Overgaard, Johannes;Kristensen, Torsten N.;Hoffmann, Ary A.
通讯作者：
Hoffmann, Ary A.
DOI：
10.1016/s1096-4959(03)00117-9
发表时间：
2003-07-01
影响因子：
2.2
作者：
Kostál, V;Berková, P;Simek, P
通讯作者：
Simek, P