Sex-specific plasma lipid profiles of ME/CFS patients and their association with pain, fatigue, and cognitive symptoms.
Sex-specific plasma lipid profiles of ME/CFS patients and their association with pain, fatigue, and cognitive symptoms.
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DOI：
10.1186/s12967-021-03035-6
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发表时间：
2021-08-28
影响因子：
7.4
通讯作者：
Abdullah L
中科院分区：
文献类型：
--
作者：
Nkiliza A;Parks M;Cseresznye A;Oberlin S;Evans JE;Darcey T;Aenlle K;Niedospial D;Mullan M;Crawford F;Klimas N;Abdullah L
肌痛性脑脊髓炎/慢性疲劳综合征（ME/CFS）是一种严重影响女性的复杂疾病。这种疾病与可能受脂质代谢影响的免疫和代谢紊乱有关。因此，我们假设ME/CFS患者的血浆脂质将提供与ME/CFS相关的免疫、炎症和代谢过程紊乱的独特生物标志物特征。对来自50名ME/CFS患者和50名对照（每组50%男性和相似的年龄和种族）的队列的血浆进行脂质组学分析。使用纳米流液相色谱（nLC）和高效液相色谱（HPLC）系统与高质量精度ORBITRAP质谱仪联用进行分析，可检测超过三个数量级的血浆脂质浓度范围。我们检测了ME/CFS患者和对照组的血浆磷脂（PL）、中性脂质（NL）和生物活性脂质，并研究了性别对脂质与ME/CFS诊断之间关系的影响。在雌性动物中，ME/CFS组的总磷脂酰乙醇胺（PE）、含omega-6花生四烯酸的PE和总己糖神经酰胺（HexCer）水平显著低于对照组。在男性中，与对照组相比，ME/CFS患者的总HexCer、单不饱和PE、磷脂酰肌醇（PI）和饱和甘油三酯（TG）水平升高。此外，与男性对照组相比，男性ME/CFS患者的omega-6亚油酸衍生的氧化脂质显著增加。主成分分析（PCA）确定了三个主要组成部分，主要包含PC和一些PE，PI和SM物种，所有这些都与头痛和疲劳的严重程度呈负相关，无论性别。氧脂素，乙醇酰胺和ME/CFS症状严重程度的相关性表明，较低浓度的这些脂质对应于头痛，疲劳和认知困难的严重程度增加，这种关联受性别的影响。在ME/CFS患者中观察到的PL、NL、HexCer和羟脂素失调的性别特异性模式表明，这些脂质可能在促进免疫功能障碍和炎症方面发挥作用，这可能是导致疲劳、慢性疼痛临床表现的潜在因素之一。和病人的认知困难。进一步评价脂质代谢途径是必要的，以更好地了解ME/CFS的发病机制。在线版本包含补充材料，可通过10.1186/s12967-021-03035-6获得。
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex illness which disproportionally affects females. This illness is associated with immune and metabolic perturbations that may be influenced by lipid metabolism. We therefore hypothesized that plasma lipids from ME/CFS patients will provide a unique biomarker signature of disturbances in immune, inflammation and metabolic processes associated with ME/CFS. Lipidomic analyses were performed on plasma from a cohort of 50 ME/CFS patients and 50 controls (50% males and similar age and ethnicity per group). Analyses were conducted with nano-flow liquid chromatography (nLC) and high-performance liquid chromatography (HPLC) systems coupled with a high mass accuracy ORBITRAP mass spectrometer, allowing detection of plasma lipid concentration ranges over three orders of magnitude. We examined plasma phospholipids (PL), neutral lipids (NL) and bioactive lipids in ME/CFS patients and controls and examined the influence of sex on the relationship between lipids and ME/CFS diagnosis. Among females, levels of total phosphatidylethanolamine (PE), omega-6 arachidonic acid-containing PE, and total hexosylceramides (HexCer) were significantly decreased in ME/CFS compared to controls. In males, levels of total HexCer, monounsaturated PE, phosphatidylinositol (PI), and saturated triglycerides (TG) were increased in ME/CFS patients compared to controls. Additionally, omega-6 linoleic acid-derived oxylipins were significantly increased in male ME/CFS patients versus male controls. Principal component analysis (PCA) identified three major components containing mostly PC and a few PE, PI and SM species—all of which were negatively associated with headache and fatigue severity, irrespective of sex. Correlations of oxylipins, ethanolamides and ME/CFS symptom severity showed that lower concentrations of these lipids corresponded with an increase in the severity of headaches, fatigue and cognitive difficulties and that this association was influenced by sex. The observed sex-specific pattern of dysregulated PL, NL, HexCer and oxylipins in ME/CFS patients suggests a possible role of these lipids in promoting immune dysfunction and inflammation which may be among the underlying factors driving the clinical presentation of fatigue, chronic pain, and cognitive difficulties in ill patients. Further evaluation of lipid metabolism pathways is warranted to better understand ME/CFS pathogenesis. The online version contains supplementary material available at 10.1186/s12967-021-03035-6.
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DOI：
10.1080/21641846.2014.978110
发表时间：
2015-01-01
期刊：
Fatigue : biomedicine, health & behavior
影响因子：
--
作者：
Jason LA;So S;Brown AA;Sunnquist M;Evans M
通讯作者：
Evans M
影响因子：
4.4
作者：
Brenu, Ekua Weba;Huth, Teilah K.;Marshall-Gradisnik, Sonya M.
通讯作者：
Marshall-Gradisnik, Sonya M.
影响因子：
--
作者：
Brenu, E W;Hardcastle, S L;Atkinson, G M;van Driel, M L;Kreijkamp-Kaspers, S;Ashton, K J;Staines, D R;Marshall-Gradisnik, S M
通讯作者：
Marshall-Gradisnik, S M
DOI：
10.3390/healthcare5020029
发表时间：
2017-06-21
期刊：
Healthcare (Basel, Switzerland)
影响因子：
--
作者：
Briggs MA;Petersen KS;Kris-Etherton PM
通讯作者：
Kris-Etherton PM
影响因子：
11
作者：
Hornig, M.;Gottschalk, G.;Lipkin, W. I.
通讯作者：
Lipkin, W. I.