Nanostructured scaffold as a determinant of stem cell fate.
Nanostructured scaffold as a determinant of stem cell fate.
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DOI：
10.1186/s13287-016-0440-y
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发表时间：
2016-12-30
影响因子：
7.5
通讯作者：
Das D
中科院分区：
文献类型：
--
作者：
Krishna L;Dhamodaran K;Jayadev C;Chatterjee K;Shetty R;Khora SS;Das D
干细胞的功能受到来自小生境的线索的严格调节，包括内在和外在细胞信号。除了化学和生长因子，生物物理信号是决定干细胞特性的外在信号的重要组成部分。支架制造中使用的材料提供化学线索，而支架的形状提供生物物理线索。支架的化学组成对干细胞命运的影响已经得到了很好的研究。生物物理信号如纳米形貌、机械力、基质硬度和生物材料粗糙度影响干细胞的命运。然而，对它们在信号传导串扰、干细胞维持和定向分化中的作用知之甚少。在各种支架设计技术中，纳米技术具有特殊的意义。纳米形貌在用于调节干细胞行为的支架设计中的作用在再生医学中已经变得重要。纳米技术允许操纵高度先进的表面/支架，以优化细胞行为的调节。技术，如静电纺丝，软光刻，微流体，碳纳米管，和纳米结构水凝胶在这篇评论中描述，沿着其在再生医学中的潜在用途。我们还简要介绍了纳米材料引发的潜在信号串扰，这些信号串扰决定了干细胞的特定结果。这篇简明的综述汇编了纳米结构的最新发展及其在指导干细胞分化用于前瞻性治疗应用中的重要性。
The functionality of stem cells is tightly regulated by cues from the niche, comprising both intrinsic and extrinsic cell signals. Besides chemical and growth factors, biophysical signals are important components of extrinsic signals that dictate the stem cell properties. The materials used in the fabrication of scaffolds provide the chemical cues whereas the shape of the scaffolds provides the biophysical cues. The effect of the chemical composition of the scaffolds on stem cell fate is well researched. Biophysical signals such as nanotopography, mechanical forces, stiffness of the matrix, and roughness of the biomaterial influence the fate of stem cells. However, not much is known about their role in signaling crosstalk, stem cell maintenance, and directed differentiation. Among the various techniques for scaffold design, nanotechnology has special significance. The role of nanoscale topography in scaffold design for the regulation of stem cell behavior has gained importance in regenerative medicine. Nanotechnology allows manipulation of highly advanced surfaces/scaffolds for optimal regulation of cellular behavior. Techniques such as electrospinning, soft lithography, microfluidics, carbon nanotubes, and nanostructured hydrogel are described in this review, along with their potential usage in regenerative medicine. We have also provided a brief insight into the potential signaling crosstalk that is triggered by nanomaterials that dictate a specific outcome of stem cells. This concise review compiles recent developments in nanoscale architecture and its importance in directing stem cell differentiation for prospective therapeutic applications.
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DOI：
10.1007/978-1-61779-953-2_15
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Collins, Maria C;Gunst, Philip R;Muller-Borer, Barbara J
通讯作者：
Muller-Borer, Barbara J
DOI：
10.1038/nrm3112
发表时间：
2011-05
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
--
作者：
Boldrin, Luisa;Elvassore, Nicola;De Coppi, Paolo
通讯作者：
De Coppi, Paolo
影响因子：
2.9
作者：
Delcroix, Gaetan. J-R.;Jacquart, Matthieu;Montero-Menei, Claudia N.
通讯作者：
Montero-Menei, Claudia N.
影响因子：
14
作者：
Chen, Ying-Chieh;Lee, Don-Ching;Chiu, Ing-Ming
通讯作者：
Chiu, Ing-Ming