Interfacing of CE in a PVP matrix to ion trap mass spectrometry: analysis of isomeric and structurally related (N-acetylamino)fluorene-modified oligonucleotides.
Interfacing of CE in a PVP matrix to ion trap mass spectrometry: analysis of isomeric and structurally related (N-acetylamino)fluorene-modified oligonucleotides.
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PVP 基质中 CE 与离子阱质谱的接口：异构体和结构相关的（N-乙酰氨基）芴修饰寡核苷酸的分析。
DOI：
10.1021/ac9713823
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发表时间：
1998
影响因子：
7.4
通讯作者：
Vouros,P
中科院分区：
文献类型：
--
作者：
Harsch,A;Vouros,P
This work demonstrates the interfacing of capillary electrophoresis in a poly(N-vinylpyrrolidone) (PVP) solution to electrospray ionization ion trap mass spectrometry (ESI-ITMS). This methodology was used for on-line analysis of modified and unmodified oligonucleotides. Oligonucleotides were covalently modified using the model carcinogen 2-(N-acetoxy-N-acetylamino)fluorene. In the presence of PVP, separation was achieved for a set of isomeric (N-acetylamino)fluorene (AAF)-modified oligonucleotides differing only in their base sequences, while open-tube control experiments showed no separation between these compounds. The resolved analytes were identified by ESI-ITMS with negative ion detection. On-line acquisition of MS and MS/MS data allowed unambiguous identification of all structural isomers. Baseline separation was also accomplished for a 10-compound mixture containing a series of five nonisomeric AAF-modified oligonucleotides and their unmodified parent oligonucleotides.
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影响凝胶填充毛细管毛细管电泳分离寡核苷酸分辨率和定量的因素
影响因子：
4.1
作者：
D. M. Demorest;R. Dubrow
通讯作者：
R. Dubrow
影响因子：
4.1
作者：
Barry, JP;Muth, J;Vouros, P
通讯作者：
Vouros, P
影响因子：
7.4
作者：
Barry, JP;Norwood, C;Vouros, P
通讯作者：
Vouros, P
影响因子：
56.9
作者：
B. Chmelka;A. Pines
通讯作者：
A. Pines
影响因子：
2.9
作者：
CERNY, RL;GROSS, ML;GROTJAHN, L
通讯作者：
GROTJAHN, L