Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses.
Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses.
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DOI：
10.1371/journal.pone.0055172
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Fredberg JJ
中科院分区：
文献类型：
--
作者：
Tambe DT;Croutelle U;Trepat X;Park CY;Kim JH;Millet E;Butler JP;Fredberg JJ
在伤口愈合、组织生长和某些癌症中，上皮或内皮单层片扩张。在膨胀的单层片内，单个细胞的迁移受到邻近细胞施加的物理力的强烈引导。这个过程被称为趋板性，是使用单层应力显微镜（MSM）发现的。然而，MSM依赖于有关边界条件、电池材料特性和系统维度的某些简化假设。为了评估这些假设的有效性，并量化相关的错误，在这里，我们报告新的分析，数值和实验研究。对于几个常用的实验单层系统，简化的假设，以前使用的误差被证明是相当小的。位移场和牵引场的平面外分量可以安全地忽略，并且作为趋性基础的细胞间应力的特征在很大程度上不受影响。总之，这些发现在广泛但明确的适用范围内验证了单层应力显微镜。
In wound healing, tissue growth, and certain cancers, the epithelial or the endothelial monolayer sheet expands. Within the expanding monolayer sheet, migration of the individual cell is strongly guided by physical forces imposed by adjacent cells. This process is called plithotaxis and was discovered using Monolayer Stress Microscopy (MSM). MSM rests upon certain simplifying assumptions, however, concerning boundary conditions, cell material properties and system dimensionality. To assess the validity of these assumptions and to quantify associated errors, here we report new analytical, numerical, and experimental investigations. For several commonly used experimental monolayer systems, the simplifying assumptions used previously lead to errors that are shown to be quite small. Out-of-plane components of displacement and traction fields can be safely neglected, and characteristic features of intercellular stresses that underlie plithotaxis remain largely unaffected. Taken together, these findings validate Monolayer Stress Microscopy within broad but well-defined limits of applicability.
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基材厚度的机械感应。
DOI：
10.1103/physreve.82.041918
发表时间：
2010-10
期刊：
Physical review. E, Statistical, nonlinear, and soft matter physics
影响因子：
--
作者：
Lin YC;Tambe DT;Park CY;Wasserman MR;Trepat X;Krishnan R;Lenormand G;Fredberg JJ;Butler JP
通讯作者：
Butler JP
影响因子：
3.8
作者：
Discher, Dennis;Dong, Cheng;Fredberg, Jeffrey J.;Guilak, Farshid;Ingber, Donald;Janmey, Paul;Kamm, Roger D.;Schmid-Schoenbein, Geert W.;Weinbaum, Sheldon
通讯作者：
Weinbaum, Sheldon
影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
DOI：
10.1073/pnas.0408482102
发表时间：
2005-02-15
影响因子：
11.1
作者：
du Roure, O;Saez, A;Ladoux, B
通讯作者：
Ladoux, B
影响因子：
4
作者：
Bagorda A;Parent CA
通讯作者：
Parent CA