Identification of oral mitis group streptococci by arbitrarily primed polymerase chain reaction.
Identification of oral mitis group streptococci by arbitrarily primed polymerase chain reaction.
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通过任意引物聚合酶链反应鉴定口腔轻症群链球菌。
DOI:
10.1034/j.1399-302x.1999.140104.x
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发表时间:
1999
影响因子:
--
通讯作者:
Larson,CJ
中科院分区:
文献类型:
--
作者:
Rudney,JD;Larson,CJ
“Mitis group” streptococci are commensal but may play some role in dental caries, septicemia or endocarditis. Rapid genotypic identification would aid studies of dental plaque ecology, or diagnostic use. AP‐PCR with 58 unpaired arbitrary primers was used to characterize 7Streptococcus gordonii, 11Streptococcus sanguis, 2Streptococcus crista, 5Streptococcus parasanguis, 18Streptococcus oralis, and 36Streptococcus mitis(22 biovar 1 and 14 biovar 2).S. parasanguis16S rRNA variable region primer RR2 produced species‐specific bands with allS. gordoniiandS. sanguis. Human Vβ1 T‐cell receptor primer 434 yielded concordant genotypic identification of all phenotypically definedS. cristaandS. parasanguis, 83% ofS. oralis, and 74% ofS. mitisbiovar 1. Amplicon patterns forS. mitisbiovar 2 were heterogeneous. Findings suggest that primers RR2 and 434 in succession will allow rapid identification of genotypic groups corresponding closely to mitis group species established by phenotype.
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影响因子:
3.4
作者:
C. Beimfohr;A. Krause;R. Amann;W. Ludwig;K. Schleifer
通讯作者:
C. Beimfohr;A. Krause;R. Amann;W. Ludwig;K. Schleifer
影响因子:
3.1
作者:
C. Ménard;C. Mouton
通讯作者:
C. Mouton
DOI:
--
发表时间:
1978
期刊:
影响因子:
--
作者:
A. Coykendall;Allan J. Munzenmaier
通讯作者:
Allan J. Munzenmaier
DOI:
--
发表时间:
1993
期刊:
影响因子:
--
作者:
A. Milnes;G. Bowden;D. Gates;R. Tate
通讯作者:
R. Tate
影响因子:
7.6
作者:
vanHoute, J;Lopman, J;Kent, R
通讯作者:
Kent, R