Clonal diversity of the taxon Porphyromonas gingivalis assessed by random amplified polymorphic DNA fingerprinting
Clonal diversity of the taxon Porphyromonas gingivalis assessed by random amplified polymorphic DNA fingerprinting
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通过随机扩增多态性 DNA 指纹分析评估牙龈卟啉单胞菌分类群的克隆多样性
DOI：
10.1128/iai.63.7.2522-2531.1995
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发表时间：
1995
影响因子：
3.1
通讯作者：
C. Mouton
中科院分区：
文献类型：
--
作者：
C. Ménard;C. Mouton
共收集到97株牙周病原体牙龈卟啉单胞菌。该收集包括具有不同临床和地理来源的人源性实验室菌株和临床分离株。生物多样性进一步增加，包括32株分离自9种不同动物的口腔。采用随机扩增多态性DNA（RAPDs）技术，用任意序列的单一引物进行PCR扩增，得到129株菌株的基因组指纹图谱。用四种九聚体寡核苷酸作为单引物，经溴化乙锭染色后，比较琼脂糖凝胶上分离的DNA产物的带型。根据主要DNA片段的位置计算距离系数，并生成树状图。我们确定了102个克隆类型（CT），可以组装成三个主要群体的聚类分析的未加权对组的方法与数学平均值。组I（n = 79个CT）包括所有97个人菌株和6个猴分离株。组II（n = 22 CT）和组III（n = 1 CT）中的菌株与组I中的菌株有很大的区别，仅包括动物来源的菌株;它们可能代表本牙龈卟啉单胞菌分类群中的两个隐藏物种。我们观察到，旧世界猴的菌株与人类基因型聚在一起，而新世界猴的菌株与动物基因型聚在一起。我们对人类菌株的研究结果还表明：（i）群体结构基本上是克隆的，（ii）没有观察到显性或广泛的CT，（iii）在特定的CT簇和宿主的牙周状况之间没有建立关系。我们的结果证实了B以前的发现。G.卢什，D. W.戴尔，T。S. Whittam，和R. K.感染（Infect. Immun. 61：204-212，1993），并建议牙龈卟啉单胞菌应被认为是作为机会性病原体的口腔寄生菌。我们的研究结果与只有少数牙龈卟啉单胞菌强毒克隆与疾病相关的假设不一致。
A total of 97 strains of the periopathogen Porphyromonas gingivalis were collected. This collection included laboratory strains and clinical isolates of human origin with diverse clinical and geographical origins. Biological diversity was further increased by including 32 strains isolated from the oral cavities of nine different animal species. Genomic fingerprints of the 129 strains were generated as random amplified polymorphic DNAs (RAPDs) by the technique of PCR amplification with a single primer of arbitrary sequence. Four nonameric oligonucleotides were used as single primers, and the banding patterns of the DNA products separated on agarose gels were compared after ethidium ethidium bromide staining. Distance coeffients based on the positions of the major DNA fragments were calculated, and dendrograms were generated. We identified 102 clonal types (CTs) that could be assembled into three main groups by cluster analysis by the unweighted pair group method with mathematic averages. Group I (n = 79 CTs) included all 97 human strains and 6 monkey isolates. The strains in group II (n = 22 CTs) and III (n = 1 CT) were strongly differentiated from those in group I and included only strains of animal origin; they likely represent two cryptic species within the present P. gingivalis taxon. We observed that strains from Old World monkeys clustered together with the human genotype, whereas strains from New World monkeys clustered with the animal genotype. Our results with human strains also indicated that (i) the population structure is basically clonal, (ii) no dominant or widespread CT could be observed, and (iii) no relationship could be established between specific clusters of CTs and the periodontal status of the host. Our results corroborate previous findings by B. G. Loos, D. W. Dyer, T. S. Whittam, and R. K. Selander (Infect. Immun. 61:204-212, 1993) and suggest that P. gingivalis should be considered a commensal of the oral cavity acting as an opportunistic pathogen. Our results are not consistent with the hypothesis that only a few virulent clones of P. gingivalis are associated with disease.
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RAPD（任意引物）PCR 在区分相关细菌菌株方面比多位点酶电泳更灵敏。
影响因子：
14.9
作者：
Wang,G;Whittam,TS;Berg,CM;Berg,DE
通讯作者：
Berg,DE
几种系统发育推断方法在不同进化速率下的一致性。
影响因子：
10.7
作者：
DeBry,RW
通讯作者：
DeBry,RW
通过多位点酶电泳区分放线杆菌（嗜血杆菌）放线菌、嗜血嗜血杆菌和副嗜血杆菌。
DOI：
10.1099/00221287-136-10-2135
发表时间：
1990
期刊：
Journal of general microbiology
影响因子：
--
作者：
Caugant,DA;Selander,RK;Olsen,I
通讯作者：
Olsen,I
六种寄生原生动物的遗传特征：随机引物 DNA 分型和多位点酶电泳之间的奇偶性。
DOI：
10.1073/pnas.90.4.1335
发表时间：
1993
影响因子：
11.1
作者：
Tibayrenc,M;Neubauer,K;Barnabé,C;Guerrini,F;Skarecky,D;Ayala,FJ
通讯作者：
Ayala,FJ
用于研究 b 型流感嗜血杆菌菌株引起的侵袭性疾病的群体遗传框架。
DOI：
10.1073/pnas.82.15.5078
发表时间：
1985
影响因子：
11.1
作者：
Musser,JM;Granoff,DM;Pattison,PE;Selander,RK
通讯作者：
Selander,RK