Synaptic organization and ionic basis of on and off channels in mudpuppy retina. I. Intracellular analysis of chloride-sensitive electrogenic properties of receptors, horizontal cells, bipolar cells, and amacrine cells.
Synaptic organization and ionic basis of on and off channels in mudpuppy retina. I. Intracellular analysis of chloride-sensitive electrogenic properties of receptors, horizontal cells, bipolar cells, and amacrine cells.
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DOI：
10.1085/jgp.67.6.639
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发表时间：
1976-06
期刊：
影响因子：
--
通讯作者：
Dacheux RF
中科院分区：
文献类型：
--
作者：
Miller RF;Dacheux RF
细胞内记录从受体，水平细胞，双极，和无长突已经进行了灌注mudpuppy眼杯。无氯（c-f）培养基的引入导致许多细胞中的初始瞬时电位变化，随后是去极化双极细胞、水平细胞和无长突细胞的去极化光诱发活性的缓慢丧失。在c-f介质中，超极化双极仍然对光刺激有反应，但拮抗性环绕机制被消除。这些效应在回到正常离子介质后是可逆的。这项研究的结果提供了深入了解视网膜连接的神经节细胞感受野组织。结论：去极化双极对on-ganglion神经节细胞具有兴奋作用，也是on-off神经节细胞的on-excitation通路。超极化双极介导关闭和开关细胞的关闭放电。无长突细胞接受来自去极化和超极化双极细胞的输入。这些发现提出了这样一种可能性，即氯离子的跨膜运动对于水平和去极化双极细胞活动的光响应是至关重要的。
Intracellular recordings from receptors, horizontal cells, bipolars, and amacrines have been carried out in the perfused mudpuppy eyecup. The introduction of a chloride-free (c-f) medium results in initial transient potential changes in many cells followed by a slow loss of light-evoked activity of the depolarizing bipolar, the horizontal cell, and the on depolarization of amacrine cells. The hyperpolarizing bipolar remains responsive to light stimulation in a c-f medium, but the antagonistic surround mechanism is abolished. These effects are reversible after returning to a normal ionic medium. The results of this study provide insight into the retinal connections which underlie ganglion cell receptive field organization. It is concluded that the depolarizing bipolar is excitatory to on ganglion cells and is also the pathway for on-excitation of on-off cells. The hyperpolarizing bipolar mediates the off discharge of off and on-off cells. Amacrine cells receive input from both depolarizing and hyperpolarizing bipolar cells. These findings raise the possibility that transmembrane movements of chloride ions are critical for the light responsiveness of horizontal and depolarizing bipolar cell activity.
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DOI：
10.1085/jgp.67.6.661
发表时间：
1976-06
期刊：
The Journal of general physiology
影响因子：
--
作者：
Miller RF;Dacheux RF
通讯作者：
Dacheux RF
影响因子：
2.5
作者：
NELSON, R
通讯作者：
NELSON, R
影响因子：
3.8
作者：
MOTOKIZAWA, F;REUBEN, JP;GRUNDFEST, H
通讯作者：
GRUNDFEST, H
影响因子：
64.8
作者：
DOWLING, JE;RIPPS, H
通讯作者：
RIPPS, H
影响因子：
5.5
作者：
BAYLOR, DA;FUORTES, MGF;OBRYAN, PM
通讯作者：
OBRYAN, PM