Identification and characterization of seminal fluid proteins in the Asian tiger mosquito, Aedes albopictus.
Identification and characterization of seminal fluid proteins in the Asian tiger mosquito, Aedes albopictus.
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DOI：
10.1371/journal.pntd.0002946
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发表时间：
2014-06
影响因子：
3.8
通讯作者：
Sirot LK
中科院分区：
文献类型：
--
作者：
Boes KE;Ribeiro JM;Wong A;Harrington LC;Wolfner MF;Sirot LK
亚洲虎蚊（白纹伊蚊）是影响人类健康的病原体的重要媒介，包括引起登革热和基孔肯雅热的病毒。它也是世界上传播最快的入侵物种之一。由于这些原因，确定控制这种蚊子的繁殖和传播的策略至关重要。在交配过程中，精液蛋白 (Sfps) 从雄性蚊子转移到雌性蚊子，这些 Sfps 以影响繁殖的方式调节雌性的行为和生理机能。尽管 Sfps 对蚊子和其他昆虫的雌性生殖行为很重要，但伊蚊中 Sfps 的身份。此前尚未有白纹伊蚊的报道。我们使用转录组学和蛋白质组学来鉴定 Ae 中的 198 Sfps。白纹伊蚊。我们讨论这些 Sfps 与 Ae 相关的可能功能。白纹伊蚊繁殖相关生物学。我们还将这些 Sfps 的序列与其他几个物种（包括伊蚊）的蛋白质（包括报道的 Sfps）进行了比较。埃及伊蚊。而 Ae 仅有 72 个（36.4%）。白纹伊蚊 Sfps 在 Ae 中具有推定的直系同源物。埃及伊蚊，表明这些物种中 Sfps 的互补序列保守性较低，但我们没有发现两个伊蚊物种之间共享的 Sfps 的进化速率升高或正选择的证据，表明这些共享的 Sfps 的序列高度保守。我们的结果为未来研究个体 Sfps 对这种蚊子的摄食和繁殖的作用奠定了基础。这些 Sfps 的功能分析可以为控制伊蚊病原体传播率的策略提供信息。白纹伊蚊。高度侵入性的亚洲虎蚊（白纹伊蚊）传播多种病原体，导致人类和其他动物患病。因此，艾.白纹伊蚊对全世界的公共卫生构成巨大且日益严重的威胁。管理该物种的繁殖和威胁的一个步骤是确定影响其繁殖生物学的因素。精液蛋白 (Sfps) 在交配过程中从雄性蚊子转移到雌性蚊子，接受 Sfps 会改变雌性的生殖行为和生理机能。在此，我们报告198 Ae的身份。白纹伊蚊 Sfps。我们讨论这些 Sfps 对繁殖的潜在作用和影响。此外，我们还比较了Ae。白纹伊蚊 Sfps 与其他物种的蛋白质（包括报道的 Sfps），包括引起人类疾病的病原体的另外两种重要蚊媒。我们的结果为未来研究单个 Sfps 对 Ae 的作用奠定了基础。白纹伊蚊的繁殖。
The Asian tiger mosquito (Aedes albopictus) is an important vector for pathogens that affect human health, including the viruses that cause dengue and Chikungunya fevers. It is also one of the world's fastest-spreading invasive species. For these reasons, it is crucial to identify strategies for controlling the reproduction and spread of this mosquito. During mating, seminal fluid proteins (Sfps) are transferred from male mosquitoes to females, and these Sfps modulate female behavior and physiology in ways that influence reproduction. Despite the importance of Sfps on female reproductive behavior in mosquitoes and other insects, the identity of Sfps in Ae. albopictus has not previously been reported. We used transcriptomics and proteomics to identify 198 Sfps in Ae. albopictus. We discuss possible functions of these Sfps in relation to Ae. albopictus reproduction-related biology. We additionally compare the sequences of these Sfps with proteins (including reported Sfps) in several other species, including Ae. aegypti. While only 72 (36.4%) of Ae. albopictus Sfps have putative orthologs in Ae. aegypti, suggesting low conservation of the complement of Sfps in these species, we find no evidence for an elevated rate of evolution or positive selection in the Sfps that are shared between the two Aedes species, suggesting high sequence conservation of those shared Sfps. Our results provide a foundation for future studies to investigate the roles of individual Sfps on feeding and reproduction in this mosquito. Functional analysis of these Sfps could inform strategies for managing the rate of pathogen transmission by Ae. albopictus. The highly invasive Asian tiger mosquito (Aedes albopictus) transmits several pathogens that cause disease in humans and other animals. Therefore, Ae. albopictus poses a large and growing threat to public health across the world. One step toward managing the reproduction and threat of this species is to determine factors that influence its reproductive biology. Seminal fluid proteins (Sfps) are transferred from male mosquitoes to females during mating, and receipt of Sfps changes female reproductive behavior and physiology. Here we report the identity of 198 Ae. albopictus Sfps. We discuss the potential roles and impacts of these Sfps on reproduction. In addition, we compare Ae. albopictus Sfps with proteins (including reported Sfps) from other species, including two other important mosquito vectors of pathogens that cause human diseases. Our results provide a foundation for future studies to investigate the roles of individual Sfps on Ae. albopictus reproduction.
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影响因子：
56.9
作者：
Ben-Shahar, Y;Robichon, A;Robinson, GE
通讯作者：
Robinson, GE
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影响因子：
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作者：
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DeSalle, Rob
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10.1098/rspb.2007.1497
发表时间：
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影响因子：
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