TACC3 promotes colorectal cancer tumourigenesis and correlates with poor prognosis.
TACC3 promotes colorectal cancer tumourigenesis and correlates with poor prognosis.
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TACC3 促进结直肠癌肿瘤发生并与不良预后相关
DOI：
10.18632/oncotarget.9628
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发表时间：
2016-07-05
期刊：
影响因子：
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通讯作者：
Liu H
中科院分区：
文献类型：
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作者：
Du Y;Liu L;Wang C;Kuang B;Yan S;Zhou A;Wen C;Chen J;Wu Y;Yang X;Feng G;Liu B;Iwamoto A;Zeng M;Wang J;Zhang X;Liu H
结直肠癌是一种恶性上皮性肿瘤，具有极强的侵袭和转移能力。转化酸性卷曲螺旋蛋白-3（TACC 3）是一种异常表达的癌基因，是多种人类肿瘤的重要生物标志物。本研究旨在探讨TACC 3在人结直肠癌中的表达及功能。我们发现，与正常对照相比，通过qRT-PCR、蛋白质印迹和免疫组织化学（IHC）染色测定，TACC 3在CRC细胞和CRC组织活检中在mRNA和蛋白质水平上均过表达。161例大肠癌组织中TACC 3的表达与临床分期（P = 0.045）、T分期（P = 0.029）和M分期（P = 0.020）显著相关。多因素分析显示TACC 3高表达是结直肠癌独立的预后指标。与TACC 3低表达的患者相比，TACC 3高表达的患者的总生存期（OS，P = 0.023）和无病生存期（DFS，P = 0.019）显著较差。此外，TACC 3敲低减弱了CRC细胞增殖、集落形成能力、迁移和侵袭能力以及裸鼠中的肿瘤发生;分别使用实时细胞分析仪（RTCA）、克隆形成分析以及transwell和异种移植物测定来测量这些特性。这些数据表明TACC 3促进CRC进展，并且可能是CRC的独立预后因子和潜在的治疗靶点。
Colorectal carcinoma (CRC) is a malignant epithelial tumour with tremendous invasion and metastatic capacity. Transforming acidic coiled-coil protein-3 (TACC3), a frequently aberrantly expressed oncogene, is an important biomarker in various human cancers. Our study aimed to investigate the expression and function of TACC3 in human CRC. We found that TACC3 was over-expressed at both the mRNA and protein levels in CRC cells and in biopsies of CRC tissues compared with normal controls as determined by qRT-PCR, western blot and immunohistochemical (IHC) staining assays. IHC staining of samples from 161 patients with CRC also revealed that TACC3 expression was significantly correlated with clinical stage (P = 0.045), T classification (P = 0.029) and M classification (P = 0.020). Multivariate analysis indicated that high TACC3 expression was an independent prognostic marker for CRC. Patients who had high TACC3 expression had significantly poorer overall survival (OS, P = 0.023) and disease-free survival (DFS, P = 0.019) compared to patients who had low TACC3 expression. Furthermore, TACC3 knockdown attenuated CRC cell proliferation, colony formation capability, migration and invasion capability, and tumourigenesis in nude mice; these properties were measured using a real-time cell analyser (RTCA), clonogenicity analysis, and transwell and xenograft assays, respectively. These data indicate that TACC3 promotes CRC progression and could be an independent prognostic factor and a potential therapeutic target for CRC.
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影响因子：
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作者：
Duncan CG;Killela PJ;Payne CA;Lampson B;Chen WC;Liu J;Solomon D;Waldman T;Towers AJ;Gregory SG;McDonald KL;McLendon RE;Bigner DD;Yan H
通讯作者：
Yan H
DOI：
10.1073/pnas.1513359112
发表时间：
2015-09-01
影响因子：
11.1
作者：
Xiong, Dan;Du, Yong;Zeng, Mu-Sheng
通讯作者：
Zeng, Mu-Sheng
影响因子：
3.7
作者：
Mahdipour M;Leitoguinho AR;Zacarias Silva RA;van Tol HT;Stout TA;Rodrigues G;Roelen BA
通讯作者：
Roelen BA
TACC3在食管鳞状细胞癌中的高表达与不良预后相关
影响因子：
--
作者：
Huang ZL;Lin ZR;Xiao YR;Cao X;Zhu LC;Zeng MS;Zhong Q;Wen ZS
通讯作者：
Wen ZS
影响因子：
11.2
作者：
LeRoy, Patrick J.;Hunter, John J.;Ecsedy, Jeffrey A.
通讯作者：
Ecsedy, Jeffrey A.