Multifunctional biodegradable polymer nanoparticles with uniform sizes: generation and in vitro anti-melanoma activity
Multifunctional biodegradable polymer nanoparticles with uniform sizes: generation and in vitro anti-melanoma activity
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尺寸均匀的多功能可生物降解聚合物纳米颗粒：生成和体外抗黑色素瘤活性
DOI：
10.1088/0957-4484/24/45/455302
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发表时间：
2013-11
期刊：
影响因子：
3.5
通讯作者：
Wu, Min
中科院分区：
文献类型：
--
作者：
Zhu, Jintao;Wang, Jing;Wu, Xian;Dong, Liyun;Deng, Renhua;Wang, Ke;Sullivan, Martin;Liu, Shanqin;Wu, Min
我们提出了一个简单的，但通用的策略，用于制造均匀的可生物降解的聚合物纳米粒子（NP）的可控尺寸的手动驱动膜挤出乳化方法。通过改变实验参数，包括初始聚合物浓度、表面活性剂浓度、挤出通过次数、膜孔径和聚合物分子量，可以容易地调节NP的尺寸和尺寸分布。此外，疏水性药物（例如，紫杉醇（PTX））和无机NP（例如，量子点（QD）和磁性NP（MNP））可以有效地并同时封装到聚合物NP中，以通过这种简单的途径形成多功能杂化NP。这些载PTX的纳米粒具有高的包封率和载药密度以及优异的药物缓释性能。作为概念证明，体外A875细胞（黑色素瘤细胞系）实验，包括通过荧光显微镜的细胞摄取分析、NP的细胞毒性分析和磁共振成像（MRI）研究，表明通过该技术产生的PTX负载的杂合NP可潜在地用作药物递送、生物成像和肿瘤治疗（包括恶性黑色素瘤治疗）的多功能递送系统。
We present a simple, yet versatile strategy for the fabrication of uniform biodegradable polymer nanoparticles (NPs) with controllable sizes by a hand-driven membrane-extrusion emulsification approach. The size and size distribution of the NPs can be easily tuned by varying the experimental parameters, including initial polymer concentration, surfactant concentration, number of extrusion passes, membrane pore size, and polymer molecular weight. Moreover, hydrophobic drugs (e.g., paclitaxel (PTX)) and inorganic NPs (e.g., quantum dots (QDs) and magnetic NPs (MNPs)) can be effectively and simultaneously encapsulated into the polymer NPs to form the multifunctional hybrid NPs through this facile route. These PTX-loaded NPs exhibit high encapsulation efficiency and drug loading density as well as excellent drug sustained release performance. As a proof of concept, the A875 cell (melanoma cell line) experiment in vitro, including cellular uptake analysis by fluorescence microscope, cytotoxicity analysis of NPs, and magnetic resonance imaging (MRI) studies, indicates that the PTX-loaded hybrid NPs produced by this technique could be potentially applied as a multifunctional delivery system for drug delivery, bio-imaging, and tumor therapy, including malignant melanoma therapy.
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影响因子：
2.2
作者：
A. Bedikian;C. Plager;N. Papadopoulos;O. Eton;J. Ellerhorst;T. Smith
通讯作者：
A. Bedikian;C. Plager;N. Papadopoulos;O. Eton;J. Ellerhorst;T. Smith
影响因子：
8.6
作者：
Xu, Zhichuan;Shen, Chengmin;Sun, Shouheng
通讯作者：
Sun, Shouheng
影响因子：
10.8
作者：
Zambaux, MF;Bonneaux, F;Vigneron, C
通讯作者：
Vigneron, C
影响因子：
3.3
作者：
Pinkernelle, J;Teichgräber, U;Bruhn, H
通讯作者：
Bruhn, H
影响因子：
17.1
作者：
Di Corato, Riccardo;Bigall, Nadja C.;Pellegrino, Teresa
通讯作者：
Pellegrino, Teresa