Single-Blinded Study Highlighting the Differences between the Small Intestines of Neonatal and Weaned Piglets.
Single-Blinded Study Highlighting the Differences between the Small Intestines of Neonatal and Weaned Piglets.
复制标题
单盲研究强调新生仔猪和断奶仔猪小肠之间的差异。
DOI：
10.3390/ani11020271
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发表时间：
2021-01-21
期刊：
影响因子：
--
通讯作者：
Yang Q
中科院分区：
文献类型：
--
作者：
Yuan C;Zhang P;Jin Y;Ullah Shah A;Zhang E;Yang Q
猪的肠道黏膜含有肠上皮和上皮下免疫细胞，形成了一种对抗微生物的屏障。尽管如此，消化道传染病仍然是养猪业中最常见和复发的疾病。肠道形态和结构的变化主要发生在出生和断奶期间。然而，新生儿和断奶仔猪肠道结构的差异尚不清楚。本研究首次评估了新生儿（0日龄）和断奶仔猪（21日龄）小肠的差异，并分析了0日龄和21日龄仔猪小肠的形态和免疫成分，为今后的机制研究提供了初步数据。肠道是机体的主要免疫结构之一，肠道黏膜包含肠上皮和上皮下免疫细胞，是引发局部免疫应答外来抗原的主要部位。猪的肠道免疫系统发育处于出生和断奶的过渡时期。本研究比较了新生仔猪和断奶仔猪小肠的形态学和免疫学差异，为预防新生仔猪肠道传染病提供了可能。断奶仔猪肠道的组织学分析显示，与新生儿相比，断奶仔猪的隐窝深度增加，IEL计数增加，回肠Peyer 's斑块增大。断奶仔猪的回肠绒毛比新生仔猪长，claudin-3蛋白表达量显著高于新生仔猪。断奶仔猪小肠中CD3+ T、杯状细胞和分泌细胞的数量也高于新生儿。断奶仔猪和新生仔猪空肠分泌iga阳性细胞数量无显著差异。断奶仔猪十二指肠和空肠大部分模式识别受体基因mRNA表达量高于新生仔猪；然而，回肠的情况正好相反。断奶仔猪空肠和回肠黏膜IL-1β和TNF-α mRNA水平高于新生仔猪。新生仔猪外周血单个核细胞中CD3+、CD4+和CD8+ T细胞显著减少。我们的研究为0日龄和21日龄仔猪小肠内不同的免疫机制提供了见解。对其他发育阶段以及小肠差异如何影响猪对病原体的反应的研究仍有必要。
The gut mucosa of pigs, which contains intestinal epithelium and subepithelial immune cells, forms a barrier against microorganisms. Nonetheless, infectious diseases of the digestive tract remain the most frequent and recurrent conditions in the swine industry. Changes in intestinal morphology and structure primarily occur at birth and during weaning. However, the difference in the intestinal structures between neonatal and weaned piglets remains unclear. In this study, for the first time, we evaluated the differences in the small intestine between neonatal (0-day-old) and weaned piglets (21-day-old) and analyzed the morphology and immunological components of the small intestines of 0- and 21-day-old piglets, thereby providing preliminary data for future mechanistic studies. The gut is one of the body’s major immune structures, and the gut mucosa, which contains intestinal epithelium and subepithelial immune cells, is the primary site for eliciting local immune responses to foreign antigens. Intestinal immune system development in pigs is a transitional period during birth and weaning. This study compares the morphological and immunological differences in the small intestine of neonatal and weaned piglets to potentially prevent intestinal infectious diseases in neonatal piglets. Histological analyses of weaned piglet intestines showed increased crypt depth, higher IEL count, and larger ileal Peyer’s patches compared with those of neonates. Additionally, the ileal villi of weaned piglets were longer than those of neonatal piglets, and claudin-3 protein expression was significantly higher in weaned than in neonatal piglets. The numbers of CD3+ T, goblet, and secretory cells were also higher in the small intestines of weaned piglets than in those of neonates. No significant differences were observed in the secretory IgA-positive cell number in the jejunum of weaned and neonatal piglets. The mRNA expression of most pattern recognition receptors genes in the duodenum and jejunum was higher in the weaned than neonatal piglets; however, the opposite was true in the ileum. The mRNA levels of IL-1β and TNF-α in the jejunal and ileal mucosa were higher in weaned piglets than in neonatal piglets. There were significantly fewer CD3+, CD4+, and CD8+ T cells from peripheral blood-mononuclear cells in neonatal piglets. Our study provides insights regarding the different immune mechanisms within the small intestines of 0- and 21-day-old piglets. Studies on the additional developmental stages and how differences in the small intestines affect the response of pigs to pathogens remain warranted.
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影响因子：
2.1
作者：
Kokuina, Elena;Breff-Fonseca, Martha C.;Mora-Diaz, Isabel
通讯作者：
Mora-Diaz, Isabel
影响因子：
7
作者：
Bailey, M;Haverson, K;Stokes, C
通讯作者：
Stokes, C
影响因子：
8
作者：
Knoop KA;Newberry RD
通讯作者：
Newberry RD
影响因子：
4.4
作者：
Basha S;Surendran N;Pichichero M
通讯作者：
Pichichero M
DOI：
10.1007/978-1-4939-3335-8_14
发表时间：
2016-01-01
期刊：
TOLL-LIKE RECEPTORS, 2 EDITION
影响因子：
--
作者：
Flaherty, Stephanie;Reynolds, Joseph M.
通讯作者：
Reynolds, Joseph M.