Resolving physical interactions between bacteria and nanotopographies with focused ion beam scanning electron microscopy.
Resolving physical interactions between bacteria and nanotopographies with focused ion beam scanning electron microscopy.
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使用聚焦离子束扫描电子显微镜解决细菌和纳米拓扑结构之间的物理相互作用。
DOI：
10.1016/j.isci.2021.102818
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发表时间：
2021-07-23
期刊：
影响因子：
5.8
通讯作者：
Su B
中科院分区：
文献类型：
--
作者：
Jenkins J;Ishak MI;Eales M;Gholinia A;Kulkarni S;Keller TF;May PW;Nobbs AH;Su B
为了更好地评估纳米形貌的抗菌机制，分析细菌-纳米形貌粘附界面是至关重要的。在这里，我们利用聚焦离子束铣削结合扫描电子显微镜生成与纳米形貌相互作用的金黄色葡萄球菌或大肠杆菌的三维重建。第一次，3D形态分析已被利用来量化每个纳米结构和细菌包膜之间的固有接触面积，提供了一个客观的框架，从中获得合成纳米形貌的可能的抗菌机制。纳米结构密度在36和58/μm2之间且尖端直径在27和50 nm之间的表面介导包膜变形和穿透，而具有较高纳米结构密度（137/μm2）的表面诱导包膜穿透和机械破裂，导致细胞体积由于胞质渗漏而显著减小。在密度为8/μm2且尖端直径大于100 nm的纳米形貌上，细菌主要粘附在纳米结构之间，导致细胞阻抗。细菌-纳米形貌相互作用可以使用FIB-SEM进行量化包膜渗透和细胞阻抗受纳米形貌密度的影响低密度纳米形貌（8个/μm2）介导细胞阻抗高密度纳米形貌（36-137个/μm2）介导变形和渗透微生物膜;表面处理
To robustly assess the antibacterial mechanisms of nanotopographies, it is critical to analyze the bacteria-nanotopography adhesion interface. Here, we utilize focused ion beam milling combined with scanning electron microscopy to generate three-dimensional reconstructions of Staphylococcus aureus or Escherichia coli interacting with nanotopographies. For the first time, 3D morphometric analysis has been exploited to quantify the intrinsic contact area between each nanostructure and the bacterial envelope, providing an objective framework from which to derive the possible antibacterial mechanisms of synthetic nanotopographies. Surfaces with nanostructure densities between 36 and 58 per μm2 and tip diameters between 27 and 50 nm mediated envelope deformation and penetration, while surfaces with higher nanostructure densities (137 per μm2) induced envelope penetration and mechanical rupture, leading to marked reductions in cell volume due to cytosolic leakage. On nanotopographies with densities of 8 per μm2 and tip diameters greater than 100 nm, bacteria predominantly adhered between nanostructures, resulting in cell impedance. Bacteria-nanotopography interactions can be quantified using FIB-SEM Envelope penetration and cell impedance are influenced by nanotopography density Low density nanotopographies (8 per μm2) mediate cell impedance High-density nanotopographies (36–137 per μm2) mediate deformation and penetration Microbiofilms; Surface treatment
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纳米结构的钛表面表现出对葡萄球菌生物膜形成的顽固性。
影响因子：
4.6
作者：
Cao Y;Su B;Chinnaraj S;Jana S;Bowen L;Charlton S;Duan P;Jakubovics NS;Chen J
通讯作者：
Chen J
影响因子：
4.6
作者：
Diu T;Faruqui N;Sjöström T;Lamarre B;Jenkinson HF;Su B;Ryadnov MG
通讯作者：
Ryadnov MG
影响因子：
16.6
作者：
Jenkins, J.;Mantell, J.;Su, B.
通讯作者：
Su, B.
影响因子：
17.1
作者：
Linklater, Denver P.;De Volder, Michael;Ivanova, Elena P.
通讯作者：
Ivanova, Elena P.
DOI：
10.1073/pnas.1916680117
发表时间：
2020-06-09
影响因子：
11.1
作者：
Ivanova, Elena P.;Linklater, Denver P.;Crawford, Russell J.
通讯作者：
Crawford, Russell J.