Nanostructured titanium surfaces exhibit recalcitrance towards Staphylococcus epidermidis biofilm formation.
Nanostructured titanium surfaces exhibit recalcitrance towards Staphylococcus epidermidis biofilm formation.
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纳米结构的钛表面表现出对葡萄球菌生物膜形成的顽固性。
DOI：
10.1038/s41598-018-19484-x
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发表时间：
2018-01-18
影响因子：
4.6
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Cao Y;Su B;Chinnaraj S;Jana S;Bowen L;Charlton S;Duan P;Jakubovics NS;Chen J
钛基植入物在医疗保健行业中无处不在，经常受到细菌附着的影响，从而导致感染。一种减少细菌生长的创新方法是在植入材料上使用纳米结构，这种结构通过细菌细胞膜的机械破裂导致接触依赖性细胞死亡。为了实现这一目标，我们利用水热处理工艺在钛表面合成了不同结构的纳米结构，然后检测了表皮葡萄球菌在这些表面上的生长情况。经2小时水热处理后的结构(称为矛型)在短时间内细菌附着最少，但在6天的时间内倾向于支持厚生物膜的形成。相比之下，经过三个小时的水热处理(称为口袋式)后获得的结构被发现可以将生物膜的形成推迟长达6天，并通过穿透或压缩缠绕在一起的纳米矛网络之间的细菌来杀死47%的初始附着的细菌。这些结果表明，袖珍纳米结构在提高对单个细菌的杀灭率方面具有有效性，并有可能推迟长期生物膜的形成。
Titanium-based implants are ubiquitous in the healthcare industries and often suffer from bacterial attachment which results in infections. An innovative method of reducing bacterial growth is to employ nanostructures on implant materials that cause contact-dependent cell death by mechanical rupture of bacterial cell membranes. To achieve this, we synthesized nanostructures with different architectures on titanium surfaces using hydrothermal treatment processes and then examined the growth of Staphylococcus epidermidis on these surfaces. The structure obtained after a two-hour hydrothermal treatment (referred to as spear-type) showed the least bacterial attachment at short times but over a period of 6 days tended to support the formation of thick biofilms. By contrast, the structure obtained after a three-hour hydrothermal treatment (referred to as pocket-type) was found to delay biofilm formation up to 6 days and killed 47% of the initially attached bacteria by penetrating or compressing the bacteria in between the network of intertwined nano-spears. The results point to the efficacy of pocket-type nanostructure in increasing the killing rate of individual bacteria and potentially delaying longer-term biofilm formation.
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影响因子：
14
作者：
Antoci, Valentin, Jr.;Adams, Christopher S.;Parvizi, Javad;Davidson, Helen M.;Composto, Russell J.;Freeman, Theresa A.;Wickstrom, Eric;Ducheyne, Paul;Jungkind, Donald;Shapiro, Irving M.;Hickok, Noreen J.
通讯作者：
Hickok, Noreen J.
影响因子：
--
作者：
Chandki R;Banthia P;Banthia R
通讯作者：
Banthia R
影响因子：
4.6
作者：
Diu T;Faruqui N;Sjöström T;Lamarre B;Jenkinson HF;Su B;Ryadnov MG
通讯作者：
Ryadnov MG
影响因子：
2.1
作者：
Chung, Kenneth K.;Schumacher, James F.;Brennana, Anthony B.
通讯作者：
Brennana, Anthony B.
影响因子：
5
作者：
Hasan, Jafar;Webb, Hayden K.;Ivanova, Elena P.
通讯作者：
Ivanova, Elena P.