PU.1 is not strictly required for B cell development and its absence induces a B-2 to B-1 cell switch.
PU.1 is not strictly required for B cell development and its absence induces a B-2 to B-1 cell switch.
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DOI：
10.1084/jem.20051089
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发表时间：
2005-11-21
期刊：
影响因子：
--
通讯作者：
Graf T
中科院分区：
文献类型：
--
作者：
Ye M;Ermakova O;Graf T
In this paper, we describe the unexpected outgrowth of B lineage cells from PU.1−/− fetal liver cultures. The cells express all early B cell genes tested, including the putative PU.1 target genes IL-7R and EBF but not B220, and can produce immunoglobulin M. However, we observed a delay in the PU.1−/− B cell outgrowth and reduced precursor frequencies, indicating that although PU.1 is not strictly required for B cell commitment, it facilitates B cell development. We also ablated PU.1 in CD19-expressing B lineage cells in vivo, using a Cre-lox approach that allows them to be tracked. PU.1 excision resulted in a shift from B-2 cells to B-1–like cells, which dramatically increased with the age of the mice. Our data indicate that this shift is predominantly caused by a B-2 to B-1 cell reprogramming. Furthermore, we found that B-2 cells express substantially more PU.1 than B-1 cells, which is consistent with the idea that maintenance of the B-2 cell phenotype requires relatively high levels of PU.1, but B-1 cells require little.
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DOI：
10.1084/jem.20050075
发表时间：
2005-05-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Dakic A;Metcalf D;Di Rago L;Mifsud S;Wu L;Nutt SL
通讯作者：
Nutt SL
影响因子：
56.9
作者：
SCOTT, EW;SIMON, MC;SINGH, H
通讯作者：
SINGH, H
影响因子：
32.4
作者：
Cariappa, A;Tang, M;Pillai, S
通讯作者：
Pillai, S
影响因子：
14.9
作者：
Rickert, RC;Roes, J;Rajewsky, K
通讯作者：
Rajewsky, K
影响因子：
32.4
作者：
Ikawa, T;Kawamoto, H;Murre, C
通讯作者：
Murre, C