Efficacy of liquid-based cytology versus conventional smears in FNA samples.
Efficacy of liquid-based cytology versus conventional smears in FNA samples.
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DOI：
10.4103/0970-9371.155225
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发表时间：
2015-01
影响因子：
1.3
通讯作者：
Ghosh JK
中科院分区：
文献类型：
--
作者：
Tripathy K;Misra A;Ghosh JK
液基细胞学(LBC)正在迅速成为评估妇科和非妇科准备的有用方法，包括细针抽吸(FNA)细胞学。均匀的细胞分布，减少血液和粘液等模糊的背景元素，保存完好的细胞核和细胞质细节，以及快速固定有助于更好地显示细胞。这项研究是为了评估液基细胞学与传统涂片在细针吸取样本中的诊断准确性。前瞻性研究110例，其中乳腺30例，淋巴结40例，唾液腺10例，甲状腺18例，骨和软组织12例。在每种情况下，都进行了两次通过。第一道次用于常规制备(CP)，第二道次产生用于薄层制备(TP)的材料。通过半定量评分系统比较CP和TP涂片在细胞密度、背景血和坏死性细胞碎片、细胞结构、信息性背景、单层细胞的存在以及核浆细节方面的差异。统计分析采用SPSS程序的Wilcoxon符号秩检验法。与CP涂片相比，LBC涂片的诊断准确性更高，这是因为缺乏背景碎片和更好的细胞形态，这是根据Wilcoxon的符号秩次检验进行的，产生的P值为<；0.001。然而，在某些情况下，由于LBC细胞大小减少、聚集和背景改变，CP的支持是必不可少的。对FNA样本进行LBC可以是一种简单而有价值的技术。只有在少数选定的病例中，背景因素是基本的诊断线索，CP和TP两者的结合是必要的。
Liquid-based cytology (LBC) is fast becoming a useful method in evaluating both gynecological and non-gynecological preparations, including fine needle aspiration (FNA) cytology. Even distribution of cells, decreasing obscuring background elements like blood and mucus, well preserved nuclear and cytoplasmic details and rapid fixation helps in better visualization of cells. This study was conducted to asses the diagnostic accuracy of liquid–based cytology versus conventional smears in fine needle aspiration samples. In this prospective study, we had 110 cases, including 30 cases of breast, 40 of lymph node, 10 of salivary glands, 18 of thyroid and 12 of bone and soft tissue. In each case, two passes were performed. The first pass was for conventional preparation (CP) and the second pass yielded material for thin-prep (TP) preparation. Both CP and TP smears were compared for cellularity, background blood and necrotic cell debris, cell architecture, informative background, presence of a monolayer of cells and nuclear and cytoplasmic details by a semiquantitative scoring system. Wilcoxon's signed rank test on an SPSS program was used for statistical analysis. Diagnostic accuracy was better in LBC smears compared with CP smears due to lack of background debris and better cell morphology, which was performed according to Wilcoxon's signed rank test, yielding a P-value of <0.001. However, in some cases, because of a decrease in cell size, clustering and altered background in LBC, a support of CP was essential. LBC performed on FNA samples can be a simple and valuable technique. Only in few selected cases, where background factor is an essential diagnostic clue, a combination of both CP and TP is necessary.
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影响因子：
1.3
作者：
Cochand-Priollet, B;Prat, JJ;Guillausseau, PJ
通讯作者：
Guillausseau, PJ
影响因子：
3.4
作者：
Chacho, MS;Mattie, ME;Schwartz, PE
通讯作者：
Schwartz, PE
影响因子：
3.4
作者：
Parfitt, Jeremy R.;McLachlin, C. Meg;Weir, Michele M.
通讯作者：
Weir, Michele M.
影响因子：
1.3
作者：
Das, D. K.;Al-Juwaiser, A.;Kapila, K.
通讯作者：
Kapila, K.
影响因子：
1.9
作者：
Saleh, Husain A.;Hammoud, Jamal;Khan, Aurang Zeb
通讯作者：
Khan, Aurang Zeb