Genomics of the honey bee microbiome.

Genomics of the honey bee microbiome.
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DOI:
10.1016/j.cois.2015.04.003
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发表时间:
2015-08-01
影响因子:
5.3
通讯作者:
Moran NA
Moran NA
中科院分区:
农林科学2区
文献类型:
--
作者:
Moran NA

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蜜蜂工蜂的肠道中含有一个独特的细菌群落。它们是微需氧的或厌氧的,早期的研究依赖于在大气氧水平下分离的实验室培养,没有明确界定。最近,这些细菌的潜在代谢和功能的更完整的图片已经成为可能,使用基于宏基因组样本的基因组方法,以及培养的分离株。其中,大多数是主机限制,通常是缺席以外的成年肠道。这些菌种包括革兰氏阴性菌群,如蜜蜂吉氏菌和苜蓿草菌,以及革兰氏阳性菌群,如某些乳杆菌和双歧杆菌菌种。这些肠道细菌物种似乎经历了长期的共同进化与蜜蜂,在某些情况下,熊蜂主机。从基因组序列预测基因功能表明在营养、消化和潜在的防御病原体中的作用。特别是,糖利用和碳水化合物分解的基因在G. apicola和乳杆菌属物种。
The guts of honey bee workers contain a distinctive community of bacterial species. They are microaerophilic or anaerobic, and were not clearly deliniated by earlier studies relying on laboratory culture of isolates under atmospheric oxygen levels. Recently, a more complete picture of the potential metabolism and functions of these bacteria has been possible, using genomic approaches based on metagenomic samples, as well as cultured isolates. Of these, most are host-restricted and are generally absent outside adult guts. These species include both Gram negative groups, such as Gilliamella apicola and Snodgrassella alvi, and Gram positive groups such as certain Lactobacillus and Bifidobacterium species. These gut bacterial species appear to have undergone long term coevolution with honey bee and, in some cases, bumble bee hosts. Prediction of gene functions from genome sequences suggests roles in nutrition, digestion, and potentially in defense against pathogens. In particular, genes for sugar utilization and carbohydrate breakdown are enriched in G. apicola and the Lactobacillus species.
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