Characterization and identification of cold tolerant near-isogenic lines in rice.
Characterization and identification of cold tolerant near-isogenic lines in rice.
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水稻耐寒近等基因系的鉴定与鉴定
DOI：
10.1270/jsbbs.62.196
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发表时间：
2012-06
期刊：
影响因子：
2.4
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Zhou L;Zeng Y;Hu G;Pan Y;Yang S;You A;Zhang H;Li J;Li Z
为了研究水稻耐冷性的遗传机制，以孕穗期最耐冷的品种昆明小白谷（KMXBG）为供体，以日本粳稻十和田（Towada）为母本，通过回交获得了耐冷近等基因系（NILs）。对5个BC 6 F5近等基因系与轮回亲本十和田在孕穗期低温处理和常温处理下的耐冷性相关性状进行了比较，结果表明，两个近等基因系仅在小穗育性相关性状上存在显著差异。对近等基因系在萌芽期、苗期和孕穗期的耐冷性分析表明，耐冷性既有相关效应，也有差异。品系1913-4和1916-1在三个生育期均表现出较强的耐性。全基因组标记筛选结果表明，遗传背景恢复比例在98%以上。KMXBG中的17个标记在两个或多个近等基因系中渐渗，这些标记区域可能存在耐冷基因。这些近等基因系是水稻品种改良和耐冷性QTL图位克隆的优良材料。
To exploit the genetic mechanism of cold tolerance in rice, cold tolerant near-isogenic lines (NILs) were developed by backcrossing Kunmingxiaobaigu (KMXBG), reported to be the most cold-tolerant variety at the booting stage, as donor, with the cold sensitive Japanese commercial japonica variety, Towada. Comparisons of cold tolerance-related traits between five BC6F5 NILs and recurrent parent Towada under cold treatment and normal temperatures at the booting stage showed that the differences between the NILs and Towada were significant only for spikelet fertility-related traits. Analyses of cold tolerance in the NILs at the budding (germination), seedling and booting stages indicated both correlated effects and differences. Lines 1913-4 and 1916-1 showed strong and stable tolerance at all three stages. Whole genome marker screening showed that the proportion of genetic background recovery was more than 98%. Seventeen markers from KMXBG were introgressed in two or more NILs, and cold tolerance genes were possibly present in these marker regions. The NILs should be excellent materials for both rice improvement and map-based cloning of cold tolerance QTLs.
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影响因子：
64.8
作者：
Matsumoto, T;Wu, JZ;Sugiura, M
通讯作者：
Sugiura, M
影响因子：
2.4
作者：
Dai, LY;Lin, XH;Zhang, DP
通讯作者：
Zhang, DP
影响因子：
5.4
作者：
Saito, K;Miura, K;Kato, A
通讯作者：
Kato, A
影响因子：
1.9
作者：
Bertin, P;Kinet, JM;Bouharmont, J
通讯作者：
Bouharmont, J
DOI：
10.1073/pnas.202336899
发表时间：
2002-10-01
影响因子：
11.1
作者：
Harr, B;Kauer, M;Schlötterer, C
通讯作者：
Schlötterer, C