A 3D-Printed, Portable, Optical-Sensing Platform for Smartphones Capable of Detecting the Herbicide 2,4-Dichlorophenoxyacetic Acid.
A 3D-Printed, Portable, Optical-Sensing Platform for Smartphones Capable of Detecting the Herbicide 2,4-Dichlorophenoxyacetic Acid.
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DOI：
10.1021/acs.analchem.7b02139
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发表时间：
2017-09-05
影响因子：
7.4
通讯作者：
Du D
中科院分区：
文献类型：
--
作者：
Wang Y;Zeinhom MMA;Yang M;Sun R;Wang S;Smith JN;Timchalk C;Li L;Lin Y;Du D
现场快速检测环境和生物样品中的除草剂和除草剂残留对于农业、环境问题、食品安全和卫生保健是重要的。传统的除草剂检测方法需要昂贵的实验室设备和较长的周转时间。在这项工作中，我们开发了一种基于智能手机的单条纹微量滴定板比色装置，用于快速和低成本的现场测试。这种便携式智能手机平台能够在一个单条纹微孔板中筛选8个样品。该装置结合了体积小（50 × 100 × 160 mm 3）和成本低（10美元）的优点。通过使用两种不同的染料溶液，即甲基蓝（MB）和罗丹明B，对红色和绿色通道校准平台。结果显示与传统实验室读数器获得的结果具有良好的相关性。我们展示了该平台用于检测除草剂2，4-二氯苯氧乙酸在1至80 ppb范围内的应用。通过我们的设备测试了自来水、大鼠血清、血浆和人血清的加标样品。使用酶标仪测定的所有加标样品的回收率为95.6%-105.2%，使用智能手机装置测定的所有样品的回收率为93.7%-106.9%。这项工作验证了智能手机光学传感平台与商业酶标仪相当;它有资格进行现场，快速和低成本的除草剂检测，用于环境评估和生物监测。
Onsite rapid detection of herbicides and herbicide residuals in environmental and biological specimens are important for agriculture, environmental concerns, food safety, and health care. The traditional method for herbicide detection requires expensive laboratory equipment and a long turnaround time. In this work, we developed a single-stripe microliter plate smartphone-based colorimetric device for rapid and low-cost in-field tests. This portable smartphone platform is capable of screening eight samples in a single-stripe microplate. The device combined the advantages of small size (50 × 100 × 160 mm3) and low cost ($10). The platform was calibrated by using two different dye solutions, i.e. methyl blue (MB) and rhodamine B, for the red and green channels. The results showed good correlation with results attained from a traditional laboratory reader. We demonstrated the application of this platform for detection of the herbicide 2,4-dichlorophenoxyacetic acid in the range of 1 to 80 ppb. Spiked samples of tap water, rat serum, plasma, and human serum were tested by our device. Recoveries obtained varied from 95.6% to 105.2% for all of the spiked samples using the microplate reader and from 93.7% to 106.9% for all of the samples using the smartphone device. This work validated that the smartphone optical-sensing platform is comparable to the commercial microplate reader; it is eligible for onsite, rapid, and low-cost detection of herbicides for environmental evaluation and biological monitoring.
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