Tandemly repeated DNA sequences from Xenopus laevis. I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat).
Tandemly repeated DNA sequences from Xenopus laevis. I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat).
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来自非洲爪蟾的串联重复 DNA 序列。
DOI：
10.1016/s0022-2836(83)80267-8
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发表时间：
1983
影响因子：
5.6
通讯作者：
Carroll,D
中科院分区：
文献类型：
--
作者：
Lam,BS;Carroll,D
在非洲爪蟾的dna中发现了一个串联重复序列家族，具有741个碱基对的基本重复单元，并指定为卫星1。除了其相当长的重复单元外，这种DNA的特征似乎与来自其他生物体的复杂卫星DNA非常相似。克隆的重复单元的核苷酸序列没有显示出更简单的内部重复的证据，也没有明显的暗示合理的RNA或蛋白质编码区。在肝脏、胚胎或卵母细胞rna中均未发现与该DNA同源的转录本。通过斑点杂交，卫星1主要以741碱基对序列的串联重复序列存在于基因组中。然而，有一些重复序列与正常序列不同（根据限制位点的缺失或增加来判断），有些重复序列的长度不同。相似的变体经常（但不总是）聚集在一起。该卫星基因组克隆的特征证实了变异的串联组织和聚类。一些变异的性质已被更详细地阐明。基本重复序列的某些区域似乎比其他区域更容易发生变异。
A family of tandemly repeated sequences, having a basic repeating unit of 741 base-pairs, has been identified inXenopus laevisDNA and designated satellite 1. Apart from its rather long repeat unit, the characteristics of this DNA appear to be quite similar to those of complex satellite DNAs from other organisms. The nucleotide sequence of a cloned repeat unit shows no evidence of simpler internal repeats, and there is no obvious suggestion of reasonable RNA- or protein-coding regions. Transcripts homologous to this DNA could not be demonstrated in liver, embryo or oocyte RNAs. By blot-hybridization, satellite 1 has been shown to exist in the genome chiefly as tandem repeats of the 741 base-pair sequence. However, there are a number of repeats that differ from the normal sequence (as judged by loss or gain of restriction sites) and some that differ in length. Similar variants are often, but not always, clustered. Characterization of genomic clones of this satellite has confirmed the tandem organization and clustering of variants. The nature of some variants has been elucidated in more detail. Some regions of the basic repeat seem to be more prone to variation than are others.
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