A country-wide study of spoligotype and drug resistance characteristics of Mycobacterium tuberculosis isolates from children in China.
A country-wide study of spoligotype and drug resistance characteristics of Mycobacterium tuberculosis isolates from children in China.
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全国儿童结核杆菌Spoligo型及耐药性特征研究
DOI：
10.1371/journal.pone.0084315
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shen AD
中科院分区：
文献类型：
--
作者：
Jiao W;Liu Z;Han R;Zhao X;Dong F;Dong H;Huang H;Tian J;Li Q;Lian L;Yin Q;Song W;Wan K;Shen AD
背景结核病（TB）仍然是人类健康的一大威胁，尤其是儿童。然而，从儿科病例中分离结核分枝杆菌培养物仍然是一个挑战。为给儿童结核病的控制提供科学依据，我们对结核分枝杆菌的基因分型及耐药特征进行了研究。中国儿科病例中的结核病分离株。方法/主要发现在这项研究中，对中国25个省份分离的总共440株菌株进行了空间寡聚分型和药敏试验，其中90株来自儿童（<15岁），159株来自青少年（15-18岁），191株来自成人（>18岁）。结果表明，北京家族菌株在中国仍占优势（在上述三组中分别为85.6%、81.1%和75.4%），尤其是在新的儿童病例中（91.0%比既往治疗病例中的69.6%，P = 0.03）。 北京基因型分离株在中国北方和中部的总收集率较高（北方为85.1%，中部为83.9%，而南方为61.6%，P<0.001），并且在所有三个年龄组中观察到类似的趋势（P = 0.708，<0.001和0.025，分别）。 在青少年中，异烟肼（INH）耐药和乙胺丁醇（EMB）耐药菌株的频率在北京菌株中显著高于非北京基因型菌株（P = 0.028 INH和P = 0.027 EMB）。 此外，在既往接受过治疗的儿童病例中，与非北京菌株相比，北京菌株对利福平（RIF）、链霉素（STR）的耐药性和多药耐药（MDR）之间存在强相关性（分别为P=0.01、0.01和0.025）。 结论北京家系在中国北方和中部的流行率高于南方，且在各年龄组均占优势。对M.从儿童中分离出的结核菌与在青少年和成人中发现的结核菌相似。北京基因型与RIF、STR和MDR耐药相关。
Background Tuberculosis (TB) is still a big threat to human health, especially in children. However, an isolation of Mycobacterium tuberculosis culture from pediatric cases remains a challenge. In order to provide some scientific basis for children TB control, we investigated the genotyping and drug resistance characteristics of M. tuberculosis isolates from pediatric cases in China. Methodology/Principal Findings In this study, a total of 440 strains including 90 from children (<15 years), 159 from adolescents (15–18 years) and 191 from adults (>18 years) isolated in 25 provinces across China were subjected to spoligotyping and drug susceptibility testing. As a result, Beijing family strains were shown to remain predominant in China (85.6%, 81.1% and 75.4% in three above groups, respectively), especially among new children cases (91.0% vs. 69.6% in previously treated cases, P = 0.03). The prevalence of the Beijing genotype isolates was higher in northern and central China in the total collection (85.1% in northern and 83.9% in central vs. 61.6% in southern China, P<0.001) and a similar trend was seen in all three age groups (P = 0.708, <0.001 and 0.025, respectively). In adolescents, the frequencies of isoniazid (INH)-resistant and ethambutol (EMB)-resistant isolates were significantly higher among Beijing strains compared to non-Beijing genotype strains (P = 0.028 for INH and P = 0.027 for EMB). Furthermore, strong association was observed between resistance to rifampicine (RIF), streptomycin (STR) and multidrug resistance (MDR) among Beijing compared to non-Beijing strains in previously treated cases of children (P = 0.01, 0.01 and 0.025, respectively). Conclusion/Significance Beijing family was more prevalent in northern and central China compared to southern China and these strains were predominant in all age groups. The genetic diversity of M. tuberculosis isolates from children was similar to that found in adolescents and adults. Beijing genotype was associated with RIF, STR and MDR resistance in previously treated children.
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影响因子：
9.4
作者：
VANSOOLINGEN, D;QIAN, LS;VANEMBDEN, JDA
通讯作者：
VANEMBDEN, JDA
影响因子：
9.4
作者：
Kamerbeek, J;Schouls, L;vanEmbden, J
通讯作者：
vanEmbden, J
影响因子：
11.8
作者：
Yang, Chongguang;Luo, Tao;Gao, Qian
通讯作者：
Gao, Qian
影响因子：
3.2
作者：
Demay, Christophe;Liens, Benjamin;Rastogi, Nalin
通讯作者：
Rastogi, Nalin
DOI：
10.5588/ijtld.10.0403
发表时间：
2011-06-01
影响因子：
4
作者：
Guo, Y-L.;Liu, Y.;Song, C-X.
通讯作者：
Song, C-X.