Mouse interferons: production by Ehrlich ascites tumour cells infected with Newcastle disease virus and its enhancement by theophylline.
Mouse interferons: production by Ehrlich ascites tumour cells infected with Newcastle disease virus and its enhancement by theophylline.
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小鼠干扰素:感染新城疫病毒的艾利希腹水肿瘤细胞产生的干扰素及其通过茶碱的增强。
DOI:
10.1099/0022-1317-49-1-91
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发表时间:
1980
期刊:
影响因子:
--
通讯作者:
Lengyel,P
中科院分区:
文献类型:
--
作者:
Slattery,E;Taira,H;Broeze,R;Lengyel,P
Conditions are described for the production of 0.3 to 0.7 NIH mouse reference standard units of interferon per cell from Ehrlich ascites tumour cells cultured as monolayers and induced by infection with Newcastle disease virus (NDV). Inclusion of theophylline (6 mm) in the medium increased the interferon yield three to four times. Cells infected with NDV started to lyse at about 15 h p.i., but infected, theophylline-treated cells lysed only 24 h p.i.Several other methylxanthines (e.g. theobromine, caffeine and isobutylmethylxanthine), when tested at concentrations similar to that of theophylline, did not boost interferon production. Dibutyryl cyclic AMP (10−6to 10−2m) did not substitute for theophylline in increasing interferon production, and, if used together with theophylline, did not cause further enhancement.
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DOI:
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发表时间:
1970
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通讯作者:
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DOI:
10.1016/s0021-9258(17)38250-9
发表时间:
1978
期刊:
The Journal of biological chemistry
影响因子:
--
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DOI:
10.1099/0022-1317-19-1-81
发表时间:
1973
期刊:
The Journal of general virology
影响因子:
--
作者:
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通讯作者:
P. Lengyel