Development of an improved polykaryon-based influenza virus rescue system.
Development of an improved polykaryon-based influenza virus rescue system.
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DOI：
10.1186/1472-6750-12-69
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发表时间：
2012-09-25
影响因子：
3.5
通讯作者：
Tiley L
中科院分区：
文献类型：
--
作者：
Bourret V;Lyall J;Ducatez MF;Guérin JL;Tiley L
从转染细胞中拯救病毒是一种非常有用的技术，可以对特定的病毒克隆进行工程设计，以实现合理的疫苗设计或基本的反向遗传学研究。然而，它常常受到初级救援成功率或产量较低的阻碍，特别是对于野外衍生的病毒株。我们研究了通过引发细胞融合来增强流感病毒拯救的可能性，以增加在同一多核体中表达所有必需质粒的机会。为此，我们使用了 Maedi-Visna 病毒包膜蛋白，该蛋白在来自多种不同物种的细胞中具有有效的融合活性。用拯救流感病毒所需的八种质粒加上表达 Maedi-Visna 病毒包膜蛋白的质粒共转染细胞，可以提高拯救效率。此外，8-质粒拯救系统的部分补充物可以转染到两个独立的细胞群中，融合后导致活病毒拯救。这里描述的简单修改有可能提高病毒拯救过程的效率并扩大反向遗传研究的潜在应用。
Virus rescue from transfected cells is an extremely useful technique that allows defined viral clones to be engineered for the purpose of rational vaccine design or fundamental reverse genetics studies. However, it is often hindered by low primary rescue success rates or yields, especially with field-derived viral strains. We investigated the possibility of enhancing influenza virus rescue by eliciting cell fusion to increase the chances of having all necessary plasmids expressed within the same polykaryon. To this end we used the Maedi-Visna Virus envelope protein which has potent fusion activity in cells from a wide range of different species. Co-transfecting cells with the eight plasmids necessary to rescue influenza virus plus a plasmid expressing the Maedi-Visna Virus envelope protein resulted in increased rescue efficiency. In addition, partial complements of the 8-plasmid rescue system could be transfected into two separate populations of cells, which upon fusion led to live virus rescue. The simple modification described here has the potential to improve the efficiency of the virus rescue process and expand the potential applications for reverse genetic studies.
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影响因子：
11.4
作者：
SCHNELL, MJ;MEBATSION, T;CONZELMANN, KK
通讯作者：
CONZELMANN, KK
影响因子：
5.4
作者：
de Wit, E;Munster, VJ;Fouchier, RAM
通讯作者：
Fouchier, RAM
影响因子：
14.9
作者：
DUNN, JJ;STUDIER, FW
通讯作者：
STUDIER, FW
DOI：
10.1098/rstb.2001.0979
发表时间：
2001-12-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Schickli, JH;Flandorfer, A;Palese, P
通讯作者：
Palese, P
影响因子：
6.4
作者：
Lipatov, AS;Webby, RJ;Webster, RG
通讯作者：
Webster, RG