Tissue culture-induced variation at simple sequence repeats in sorghum (Sorghum bicolor L.) is genotype-dependent and associated with down-regulated expression of a mismatch repair gene, MLH3
Tissue culture-induced variation at simple sequence repeats in sorghum (Sorghum bicolor L.) is genotype-dependent and associated with down-regulated expression of a mismatch repair gene, MLH3
复制标题
高粱 (Sorghum bicolor L.) 中组织培养诱导的简单序列重复变异具有基因型依赖性，并与错配修复基因 MLH3 的表达下调相关
DOI：
10.1007/s00299-009-0797-9
复制
发表时间：
2009
影响因子：
6.2
通讯作者：
Liu B.
中科院分区：
文献类型：
--
作者：
Wang H.;Xu K.;Zhang M.;Qi B.;Dong Z.;Liu B.
体细胞无性系变异是植物组织培养中的一种常见现象。微卫星或简单重复序列（SSR）是真核生物基因组中普遍存在的组成部分，并且在包括组织培养在内的各种胁迫条件下本质上是不稳定的。在这里，我们评估了一组29个映射SSR位点的愈伤组织和再生植株来自一对互惠高粱株间F1杂种和他们的纯系亲本的遗传稳定性。我们进一步测量了一组9错配修复（MMR）编码基因和DEMETER（DME），DNA糖基化酶结构域蛋白编码基因在这些线的稳态成绩单，并测试了一个给定的MMR或DME基因和SSR变异的表达改变之间的可能关系。结果表明，两个纯系的愈伤组织和再生植株中均存在SSR变异，但变异频率差异较大（20.7%对6.9%），而F1代的愈伤组织和再生植株中未检测到SSR变异。与供体种子植物相比，所有9个研究的MMR基因和DME基因的表达显着改变，观察愈伤组织中，更显着，在再生植株。然而，只有一个基因，即，MLH3的基因型特异性表达模式发生改变，并与SSR不稳定性的发生显著相关。
Somaclonal variation is a common phenomenon associated with plant tissue culture. Microsatellites or simple sequence repeats (SSRs) are ubiquitous components of eukaryotic genomes, and are intrinsically unstable under various stress conditions including tissue culture. Here, we assessed genetic stability of a set of 29 mapped SSR loci in calli and regenerated plants derived from a pair of reciprocal sorghum inter-strain F1 hybrids and their pure line parents. We further measured the steady-state transcripts of a set of nine mismatch repair (MMR)-encoding genes and a DEMETER (DME), a DNA glycosylase domain protein-encoding gene in these lines, and tested for a possible relationship between altered expression of a given MMR or DME gene and the SSR variations. We found that SSR variations occurred in calli and regenerated plants of both the studied pure lines though at sharply different frequencies (20.7 vs. 6.9%), but no variation was detected in calli and regenerated plants of the pair of F1 hybrids. Compared with the donor seed plants, markedly altered expression of all nine studied MMR genes and the DME gene was observed in calli, and more conspicuously, in the regenerated plants. However, only one gene, i.e., MLH3, showed an altered expression pattern that is genotype specific and significantly correlated with the occurrence of SSR instability.
登录
查看更多内容
影响因子：
14.9
作者：
D. Nag;Manisha Suri;E. Stenson
通讯作者：
D. Nag;Manisha Suri;E. Stenson
影响因子：
9.8
作者：
Tanurdzic M;Vaughn MW;Jiang H;Lee TJ;Slotkin RK;Sosinski B;Thompson WF;Doerge RW;Martienssen RA
通讯作者：
Martienssen RA
影响因子：
14.9
作者：
Shiau-Yin Wu;K. Culligan;M. Lamers;J. Hays
通讯作者：
Shiau-Yin Wu;K. Culligan;M. Lamers;J. Hays
影响因子：
2.1
作者：
T. Ryu;S. Yi;Yong-Sham Kwon;Byung-Dong Kim
通讯作者：
T. Ryu;S. Yi;Yong-Sham Kwon;Byung-Dong Kim
影响因子：
6.2
作者：
L. Marum;M. Rocheta;J. Marôco;M. Oliveira;C. Miguel
通讯作者：
L. Marum;M. Rocheta;J. Marôco;M. Oliveira;C. Miguel