Eph/Ephrin signalling maintains eye field segregation from adjacent neural plate territories during forebrain morphogenesis.
Eph/Ephrin signalling maintains eye field segregation from adjacent neural plate territories during forebrain morphogenesis.
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DOI：
10.1242/dev.097048
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Wilson SW
中科院分区：
文献类型：
--
作者：
Cavodeassi F;Ivanovitch K;Wilson SW
在前脑的形态发生过程中，形成眼睛、端脑和间脑的细胞发生了广泛的重组。关于调节区域特异性行为和维持经历特定形态发生过程的细胞群体的一致性的分子机制知之甚少。在这项研究中，我们表明，在斑马鱼前脑形态发生的早期阶段，Eph/Ephrin信号通路的活动保持隔离之间的前瞻性的眼睛和相邻区域的前神经板。几个Ephrin和Ephs的互补结构域中表达的前瞻性前脑和组合废除其活动的结果在不完全分离的眼睛和端脑和缺陷外翻的视泡。相反，外源性Ephs或Ephrin在预期前脑的区域中的表达改变了细胞的粘附特性，导致分离到错误的结构域而不改变其区域命运。在rx 3突变体的眼睛形态发生的失败是伴随着Ephs和Ephrin的互补表达的损失，这表明该途径被激活的区域命运规范机制的下游，以建立域之间的边界经历不同的程序形态发生。
During forebrain morphogenesis, there is extensive reorganisation of the cells destined to form the eyes, telencephalon and diencephalon. Little is known about the molecular mechanisms that regulate region-specific behaviours and that maintain the coherence of cell populations undergoing specific morphogenetic processes. In this study, we show that the activity of the Eph/Ephrin signalling pathway maintains segregation between the prospective eyes and adjacent regions of the anterior neural plate during the early stages of forebrain morphogenesis in zebrafish. Several Ephrins and Ephs are expressed in complementary domains in the prospective forebrain and combinatorial abrogation of their activity results in incomplete segregation of the eyes and telencephalon and in defective evagination of the optic vesicles. Conversely, expression of exogenous Ephs or Ephrins in regions of the prospective forebrain where they are not usually expressed changes the adhesion properties of the cells, resulting in segregation to the wrong domain without changing their regional fate. The failure of eye morphogenesis in rx3 mutants is accompanied by a loss of complementary expression of Ephs and Ephrins, suggesting that this pathway is activated downstream of the regional fate specification machinery to establish boundaries between domains undergoing different programmes of morphogenesis.
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影响因子：
9.2
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